Scientific article

The Th2 lymphoproliferation developing in LatY136F mutant mice triggers polyclonal B cell activation and systemic autoimmunity

Published inThe Journal of immunology, vol. 177, no. 4, p. 2285-2293
Publication date2006

Lat(Y136F) knock-in mice harbor a point mutation in Tyr(136) of the linker for activation of T cells and show accumulation of Th2 effector cells and IgG1 and IgE hypergammaglobulinemia. B cell activation is not a direct effect of the mutation on B cells since in the absence of T cells, mutant B cells do not show an activated phenotype. After adoptive transfer of linker for activation of T cell mutant T cells into wild-type, T cell-deficient recipients, recipient B cells become activated. We show in vivo and in vitro that the Lat(Y136F) mutation promotes T cell-dependent B cell activation leading to germinal center, memory, and plasma cell formation even in an MHC class II-independent manner. All the plasma and memory B cell populations found in physiological T cell-dependent B cell responses are found. Characterization of the abundant plasmablasts found in secondary lymphoid organs of Lat(Y136F) mice revealed the presence of a previously uncharacterized CD93-expressing subpopulation, whose presence was confirmed in wild-type mice after immunization. In Lat(Y136F) mice, B cell activation was polyclonal and not Ag-driven because the increase in serum IgG1 and IgE concentrations involved Abs and autoantibodies with different specificities equally. Although the noncomplement-fixing IgG1 and IgE are the only isotypes significantly increased in Lat(Y136F) serum, we observed early-onset systemic autoimmunity with nephritis showing IgE autoantibody deposits and severe proteinuria. These results show that Th2 cells developing in Lat(Y136F) mice can trigger polyclonal B cell activation and thereby lead to systemic autoimmune disease.

  • Adaptor Proteins, Signal Transducing/genetics/*physiology
  • *Amino Acid Substitution/genetics
  • Animals
  • Autoimmune Diseases/*genetics/*immunology/pathology
  • B-Lymphocytes/cytology/*immunology/pathology
  • Clone Cells
  • Lymphocyte Activation/*genetics
  • Lymphoproliferative Disorders/*genetics/immunology
  • Membrane Proteins/genetics/*physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Mutant Strains
  • Phenylalanine/genetics
  • Phosphoproteins/genetics/*physiology
  • Th2 Cells/immunology/*pathology
  • Tyrosine/genetics
Affiliation Not a UNIGE publication
Citation (ISO format)
GENTON, Celine et al. The Th2 lymphoproliferation developing in LatY136F mutant mice triggers polyclonal B cell activation and systemic autoimmunity. In: The Journal of immunology, 2006, vol. 177, n° 4, p. 2285–2293. doi: 10.4049/jimmunol.177.4.2285
Updates (1)
ISSN of the journal0022-1767

Technical informations

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