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Scientific article
English

Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

Published inEMBO journal, vol. 19, no. 24, p. 6697-6703
Publication date2000
Abstract

Escherichia coli K-12, the most widely used laboratory bacterium, does not secrete proteins into the extracellular medium under standard growth conditions, despite possessing chromosomal genes encoding a putative type II secretion machinery (secreton). We show that in wild-type E.coli K-12, divergent transcription of the two operons in the main chromosomal gsp locus, encoding the majority of the secreton components, is silenced by the nucleoid-structuring protein H-NS. In mutants lacking H-NS, the secreton genes cloned on a moderate-copy-number plasmid are expressed and promote efficient secretion of the endogenous, co-regulated endochitinase ChiA. This is the first time that secretion of an endogenous extracellular protein has been demonstrated in E.coli K-12.

Keywords
  • Bacterial Proteins/ genetics/secretion
  • Base Sequence
  • Chitinase
  • Chromosome Mapping
  • Escherichia coli/ enzymology/ genetics
  • Gene Expression Regulation, Bacterial
  • Gene Expression Regulation, Enzymologic
  • Molecular Sequence Data
  • Operon
  • Plasmids
  • Transcription, Genetic
Affiliation Not a UNIGE publication
Citation (ISO format)
FRANCETIC, O. et al. Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. In: EMBO journal, 2000, vol. 19, n° 24, p. 6697–6703. doi: 10.1093/emboj/19.24.6697
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ISSN of the journal0261-4189
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