Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

Francetic, O.; Belin, Dominique; Badaut, C.; Pugsley, A. P.

doi:10.1093/emboj/19.24.6697

Scientific article

English

Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

Contributors

Francetic, O.; Belin, Dominique; Badaut, C.; Pugsley, A. P.

Published in

EMBO journal, vol. 19, no. 24, p. 6697-6703

Publication date

2000

Abstract

Escherichia coli K-12, the most widely used laboratory bacterium, does not secrete proteins into the extracellular medium under standard growth conditions, despite possessing chromosomal genes encoding a putative type II secretion machinery (secreton). We show that in wild-type E.coli K-12, divergent transcription of the two operons in the main chromosomal gsp locus, encoding the majority of the secreton components, is silenced by the nucleoid-structuring protein H-NS. In mutants lacking H-NS, the secreton genes cloned on a moderate-copy-number plasmid are expressed and promote efficient secretion of the endogenous, co-regulated endochitinase ChiA. This is the first time that secretion of an endogenous extracellular protein has been demonstrated in E.coli K-12.

Keywords

Bacterial Proteins/ genetics/secretion
Base Sequence
Chitinase
Chromosome Mapping
Escherichia coli/ enzymology/ genetics
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Molecular Sequence Data
Operon
Plasmids
Transcription, Genetic

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

FRANCETIC, O. et al. Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. In: EMBO journal, 2000, vol. 19, n° 24, p. 6697–6703. doi: 10.1093/emboj/19.24.6697

Article

Identifiers

PID : unige:11257
DOI : 10.1093/emboj/19.24.6697
PMID : 11118204

Additional URL for this publication

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC305903/pdf/cdd676.pdf

Journal ISSN

0261-4189

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27/08/2010 13:34:31

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14/03/2023 16:03:18

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29/10/2024 16:53:52

Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

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Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

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Additional URL for this publication

Journal ISSN

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Creation

First validation

Update

Status update

Last indexation