Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells

Goujon, Antoine; Strakova, Karolina; Sakai, Naomi; Matile, Stefan

doi:10.1039/C8SC03620A

Scientific article

English

Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells

Contributors

Goujon, Antoine; Strakova, Karolina; Sakai, Naomi

; Matile, Stefan

Published in

Chemical Science, vol. 10, no. 1, p. 310-319

Publication date

2019

Abstract

To image the mechanical properties of biological membranes, twisted push–pull mechanophores that respond to membrane tension by planarization in the ground state have been introduced recently. For their application in biological systems, these so-called fluorescent flippers will have to be localized to specific environments of cellular membranes. In this report, we explore streptavidin as a versatile connector between biotinylated flipper probes and biotinylated targets. Fluorescence spectroscopy and microscopy with LUVs and GUVs reveal the specific conditions needed for desthiobiotin-loaded streptavidin to deliver biotinylated flippers selectively to biotinylated membranes. Selectivity for biotinylated plasma membranes is also observed in HeLa cells, confirming the compatibility of this strategy with biological systems. Streptavidin interfacing does not affect the mechanosensitivity of the flipper probes, red shift in the excitation maximum and fluorescence lifetime increase with membrane order and tension, as demonstrated, inter alia, using FLIM.

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

UNIGE research groups

Groupe Matile

Citation (ISO format)

GOUJON, Antoine et al. Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells. In: Chemical Science, 2019, vol. 10, n° 1, p. 310–319. doi: 10.1039/C8SC03620A

Article (Published version)

CC BY

Identifiers

PID : unige:112559
DOI : 10.1039/C8SC03620A

Additional URL for this publication

http://xlink.rsc.org/?DOI=C8SC03620A

Journal ISSN

2041-6520

665views

210downloads

Creation

02/01/2019 11:00:00

First validation

02/01/2019 11:00:00

Update

13/10/2025 18:36:29

Status update

15/03/2023 15:19:40

Last indexation

16/02/2026 06:58:06

Streptavidin interfacing as a general strategy to localize fluorescent membrane tension probes in cells

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation