CD4+ T cells play a major role for IgM and IgG anti-DNA production in mice infected with Plasmodium yoelii

Fossati-Jimack, Liliane; Merino, Jesus; Izui, Shozo

doi:10.1111/j.1365-2249.1990.tb05193.x

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Title		CD4+ T cells play a major role for IgM and IgG anti-DNA production in mice infected with Plasmodium yoelii
Authors		Fossati-Jimack, Liliane Merino, Jesus Izui, Shozo
Published in		Clinical and Experimental Immunology. 1990, vol. 79, no. 2, p. 291-296
Abstract		The infection by a non-lethal strain of Plasmodium yoelii induces the formation of autoantibodies such as anti-DNA and anti-Sm antibodies in mice. The extent of the relative increase in serum levels of IgM and IgG anti-DNA and anti-Sm antibodies and their kinetics were found to be similar to those of anti-hapten antibodies and of total IgM and IgG levels. This strongly suggested that anti-DNA and anti-Sm autoantibody responses observed in malaria-infected mice are a result of polyclonal activation of B cells. The analysis of the IgG subclasses reacting with DNA antigen showed significant levels of the T cell-dependent isotypes, IgG1 and IgG2. The role of T cells in the activation of autoreactive B cells was confirmed by using athymic nude mice. Indeed, BALB/c-nu/nu and C57BL/6-nu/nu mice failed to produce IgG anti-DNA antibodies after infection with P. yoelii. Moreover, the reconstitution of BALB/c nude mice with lymph node cells from congenic euthymic BALB-Igb mice showed the activation of autoreactive B cells in nude mice by T cells from euthymic mice. Studies in mice depleted of CD4+ T cells strongly suggested that malaria-induced anti-DNA antibodies were almost entirely dependent on the presence of CD4+ T cells, as this depletion significantly decreased IgM anti-DNA antibodies and completely abolished the IgG anti-DNA production, including the IgG3 subclass in infected mice. In contrast, depletion of the CD8+ T cell subset had no effect on the production of autoantibody in malaria-infected mice. Our results indicate that CD4+ T cells play a major role for both IgM and IgG anti-DNA production during the course of malaria infection.
Keywords		Animals — Antibodies, Antinuclear/ biosynthesis — Antibodies, Protozoan/biosynthesis — Autoantigens/immunology — CD4-Positive T-Lymphocytes/immunology/ physiology — Female — Immunoglobulin G/ biosynthesis — Immunoglobulin M/ biosynthesis — Malaria/ immunology — Mice — Mice, Inbred BALB C — Mice, Inbred C57BL — Mice, Nude — Plasmodium yoelii/immunology — Ribonucleoproteins/immunology — Ribonucleoproteins, Small Nuclear — snRNP Core Proteins
Identifiers		DOI: 10.1111/j.1365-2249.1990.tb05193.x PMID: 1968798
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1534765/pdf/clinexpimmunol00077-0153.pdf
Citation (ISO format)		FOSSATI-JIMACK, Liliane, MERINO, Jesus, IZUI, Shozo. CD4+ T cells play a major role for IgM and IgG anti-DNA production in mice infected with Plasmodium yoelii. In: Clinical and Experimental Immunology, 1990, vol. 79, n° 2, p. 291-296. doi: 10.1111/j.1365-2249.1990.tb05193.x https://archive-ouverte.unige.ch/unige:11250