Scientific article

In vivo myocardial infarct area at risk assessment in the rat using manganese enhanced magnetic resonance imaging (MEMRI) at 1.5T

Published inMagnetic resonance in medicine, vol. 59, no. 6, p. 1422-1430
Publication date2008

The aim of this study was to measure the myocardial area at risk in rat, using MRI and manganese injection during a coronary occlusion/reperfusion model at 1.5T. A sequential protocol with occlusion and MnCl2 injection immediately followed by MRI was used with the assumption that MnCl2-induced contrast persistence is enough to accurately image the area at risk 90 min after occlusion. A total of 15 adult rats underwent a single 30-min episode of coronary occlusion followed by reperfusion. MnCl2 was injected (25 micromol/kg) at the beginning of the occlusion for 11 rats (group 1) and 6 h after reperfusion for four animals (group 2). A deficit of signal enhancement was observed in all rats. Hypoenhancement area in group 1 was correlated to the area at risk delineated by methylene blue (r=0.96, P<0.0001) whereas in group 2 it was correlated to the infarct area given by triphenyltetrazolium chloride (TTC) solution (r=0.98, P=0.003). The area at risk size was significantly correlated with left ventricle ejection fraction (LVEF), end-systolic volume and anterolateral wall thickening. This work demonstrates that hypoenhanced zone obtained after manganese injection during occlusion represents the area at risk and not only the infarct zone.

  • Analysis of Variance
  • Animals
  • Chlorides/diagnostic use
  • Contrast Media
  • Disease Models, Animal
  • Electrocardiography
  • Image Enhancement/methods
  • Image Processing, Computer-Assisted/methods
  • Magnetic Resonance Imaging/methods
  • Manganese Compounds/diagnostic use
  • Myocardial Infarction/pathology
  • Rats
  • Rats, Sprague-Dawley
  • Risk Assessment
Citation (ISO format)
DAIRE, Jean-Luc et al. In vivo myocardial infarct area at risk assessment in the rat using manganese enhanced magnetic resonance imaging (MEMRI) at 1.5T. In: Magnetic resonance in medicine, 2008, vol. 59, n° 6, p. 1422–1430. doi: 10.1002/mrm.21493
Main files (1)
Article (Accepted version)
ISSN of the journal0740-3194

Technical informations

Creation03/11/2009 10:03:00 AM
First validation03/11/2009 10:03:00 AM
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