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Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau

Rodrigues, Vinícius Peixoto
Rocha, Cláudia Quintino da
Périco, Larissa Lucena
Santos, Raquel de Cássia Dos
Ohara, Rie
Nishijima, Catarine Massucato
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Published in International Journal of Molecular Sciences. 2017, vol. 18, no. 11
Abstract Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as "cipó-una", it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief.
Keywords Acid Sensing Ion Channels/metabolismAnalgesics/chemistry/isolation & purification/pharmacology/therapeutic useAnimalsBignoniaceae/chemistryLocomotion/drug effectsMaleMicePain/drug therapy/metabolismPhytotherapyPlant Extracts/chemistry/isolation & purification/pharmacology/therapeutic usePlant Roots/chemistryPlantsMedicinal/chemistryTRPM Cation Channels/metabolism
PMID: 29099043
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Research group Phytochimie et produits naturels bioactifs
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RODRIGUES, Vinícius Peixoto et al. Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau. In: International Journal of Molecular Sciences, 2017, vol. 18, n° 11. doi: 10.3390/ijms18112304 https://archive-ouverte.unige.ch/unige:112402

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Deposited on : 2018-12-18

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