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Scientific article
English

Actin isoforms in amphioxus Branchiostoma lanceolatum

Contributors

Fagotti, A.; Di Rosa, I.; Simoncelli, F.; Chaponnier, Christine; Gabbiani, Giulio; Pascolini, R.

Published in

Cell and tissue research, vol. 292, no. 1, p. 173-176

Publication date

1998

Abstract

Actin is a highly conserved cytoskeletal protein that is ubiquitous in all eukaryotes. Little is known about actin expression in amphioxus, the closest living relative of the vertebrates. In the present study, involving Western blotting and indirect immunofluorescence, we report the characterization and localization of various actin isoforms in amphioxus (Branchiostoma lanceolatum) tissues. Three antibodies against vertebrate actins were used: a polyclonal antibody recognizing beta-cytoplasmic actin (anti-beta actin), a monoclonal antibody against sarcomeric actins (anti-alphaSR-1), and a monoclonal antibody specific for alpha-smooth actin (anti-alphaSM-1). Western blot analysis of amphioxus extracts immunodecorated with these antibodies showed a 43-kDa-positive band co-migrating with respective controls. The amphioxus isoactin expression patterns recognized by these antibodies were similar to those of vertebrates, i.e., anti-beta actin showed positive staining mainly in non-muscle cells, anti-alphaSR-1 labelled dorsolateral myotomal muscles, and anti-alphaSM-1 stained ventral muscles. These results demonstrate that at least two muscle actins are present in amphioxus, suggesting that muscle actin gene duplication events began before vertebrate divergence from the amphioxus lineage.

Keywords

  • Actins/ analysis/biosynthesis
  • Animals
  • Blotting, Western
  • Chordata, Nonvertebrate/ chemistry
  • Fluorescent Antibody Technique, Indirect
  • Isomerism

UNIGE affiliation entities

Citation (ISO format)

FAGOTTI, A. et al. Actin isoforms in amphioxus Branchiostoma lanceolatum. In: Cell and tissue research, 1998, vol. 292, n° 1, p. 173–176. doi: 10.1007/s004410051047

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Additional URL for this publication

http://www.springerlink.com/content/wbknubex4u4m12tr/fulltext.pdf

Journal ISSN

0302-766X
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27/08/2010 13:34:25

First validation

27/08/2010 13:34:25

Update

14/03/2023 16:03:11

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14/03/2023 16:03:11

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