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Title

Quantification of T-cell progenitors during ontogeny: thymus colonization depends on blood delivery of progenitors

Authors
Dunon, D.
Allioli, N.
Vainio, O.
Published in Blood. 1999, vol. 93, no. 7, p. 2234-2243
Abstract An in vivo thymus reconstitution assay based on intrathymic injection of hematopoietic progenitors into irradiated chicks was used to determine the number of T-cell progenitors in peripheral blood, paraaortic foci, bone marrow (BM), and spleen during ontogeny. This study allowed us to analyze the regulation of thymus colonization occurring in three waves during embryogenesis. It confirmed that progenitors of the first wave of thymus colonization originate from the paraaortic foci, whereas progenitors of the second and the third waves originate from the BM. The analysis of the number of T-cell progenitors indicates that each wave of thymus colonization is correlated with a peak number of T-cell progenitors in peripheral blood, whereas they are almost absent during the periods defined as refractory for colonization. Moreover, injection of T-cell progenitors into the blood circulation showed that they homed into the thymus without delay during the refractory periods. Thus, thymus colonization kinetics depend mainly on the blood delivery of T-cell progenitors during embryogenesis.
Keywords AnimalsBlood Cells/ cytologyBone Marrow/embryologyCell DifferentiationCell LineageCell MovementChick Embryo/cytology/ growth & developmentHematopoietic Stem Cell TransplantationLymph Nodes/ cytology/embryologyRadiation ChimeraSpleen/cytology/embryologyT-Lymphocytes/ cytologyThymus Gland/ cytology/embryology
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PMID: 10090932
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DUNON, D. et al. Quantification of T-cell progenitors during ontogeny: thymus colonization depends on blood delivery of progenitors. In: Blood, 1999, vol. 93, n° 7, p. 2234-2243. https://archive-ouverte.unige.ch/unige:11227

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Deposited on : 2010-08-27

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