Scientific article
Open access

Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity

Published inJournal of cell science, vol. 122, no. Pt 16, p. 2980-2988
Publication date2009

Using newly generated monoclonal antibodies, we have compared the distribution of beta- and gamma-cytoplasmic actin in fibroblastic and epithelial cells, in which they play crucial roles during various key cellular processes. Whereas beta-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, gamma-actin displays a more versatile organization, according to cell activities. In moving cells, gamma-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility; in stationary cells, gamma-actin is also recruited into stress fibers. beta-actin-depleted cells become highly spread, display broad protrusions and reduce their stress-fiber content; by contrast, gamma-actin-depleted cells acquire a contractile phenotype with thick actin bundles and shrinked lamellar and lamellipodial structures. Moreover, beta- and gamma-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion. Our results reveal new aspects of beta- and gamma-actin organization that support their functional diversity.

  • Actins/chemistry/ metabolism
  • Actomyosin/metabolism
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Cell Compartmentation
  • Cell Movement
  • Cell Shape
  • Cytoplasm/ metabolism
  • Epithelial Cells/cytology/metabolism
  • Fibroblasts/cytology/metabolism
  • Gene Silencing
  • Humans
  • Mitosis
  • Models, Biological
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins/antagonists & inhibitors
  • Protein Transport
  • Pseudopodia/metabolism
  • Rats
  • Subcellular Fractions/metabolism
  • Sus scrofa
Citation (ISO format)
DUGINA, Vera et al. Beta and gamma-cytoplasmic actins display distinct distribution and functional diversity. In: Journal of cell science, 2009, vol. 122, n° Pt 16, p. 2980–2988. doi: 10.1242/jcs.041970
Updates (1)
ISSN of the journal0021-9533

Technical informations

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