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Scientific Article
unige:11214 
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Functional analysis of T cell subsets from mice bearing the lpr gene |
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| Authors | ||
| Published in | Journal of Immunology. 1985, vol. 135, no. 4, p. 2423-2428 | |
| Abstract | The autosomal recessive lpr (lymphoproliferation) gene is responsible for a thymus-dependent massive lymphoproliferation associated with the development of lupus-like autoimmune disease. Phenotypic analysis of adult lpr/lpr lymph nodes has demonstrated accumulation of a dull Lyt-1+, Thy-1+ population that expresses neither Lyt-2 nor L3T4 antigens. With the use of a depletion method based on complement-mediated lysis with an anti-Lyt-2 monoclonal antibody (31 M) and a new anti-L3T4 monoclonal antibody (RL 172.4), we have purified the Lyt-2- L3T4- subset from lymph nodes or spleens of C57BL/6-lpr/lpr mice and determined whether they are immunologically functional in vitro. Production of neither interleukin 2 nor interferon-gamma was detected by the double-negative subset after stimulation with concanavalin A and/or phorbol myristate acetate. The frequencies of allospecific cytotoxic T lymphocyte (CTL) precursors and lectin-induced antigen-nonspecific CTL precursors were diminished to almost undetectable levels, whereas the Lyt-2+ population from lpr/lpr mice had CTL-precursor frequencies comparable with that of +/+ mice. These results show that the major cell subset of adult lpr/lpr lymph nodes or spleens is composed of lymphocytes with markedly limited potential for lymphokine production or antigenic stimulation. | |
| Keywords | Animals — Antigens, Ly — Antigens, Surface/analysis — Cell Separation — Concanavalin A/pharmacology — Cytotoxicity, Immunologic — Female — Flow Cytometry — Genes, Recessive — Interferon-gamma/biosynthesis — Interleukin-2/biosynthesis — Isoantigens/immunology — Lymphocyte Activation — Male — Mice — Mice, Inbred C3H — Mice, Inbred C57BL — Mice, Inbred DBA — Mice, Mutant Strains/ immunology — Phenotype — T-Lymphocytes/ classification/immunology — T-Lymphocytes, Cytotoxic/immunology | |
| Identifiers | PMID: 3928747 | |
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| Citation (ISO format) | DAVIGNON, J. L. et al. Functional analysis of T cell subsets from mice bearing the lpr gene. In: Journal of Immunology, 1985, vol. 135, n° 4, p. 2423-2428. https://archive-ouverte.unige.ch/unige:11214 |
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