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Title

Angiotensin II stimulates alpha-skeletal actin expression in cadiomyocytes in vitro and in vivo in the absence of hypertension
Authors
Clement, Sophie
Pellieux, C.
Chaponnier, Christine
Pedrazzini, T.
Gabbiani, Giulio
Published in Differentiation. 2001, vol. 69, no. 1, p. 66-74
Abstract Using a specific alpha-skeletal actin antibody, we have previously shown, that during hypertension-associated cardiac hypertrophy in the rat, the expression of alpha-skeletal actin in the myocardium is increased, but maintains focal distribution, compared to normotensive animals. In the present study, we have investigated whether alpha-skeletal actin expression can be induced in the absence of hypertension. For this purpose, we have examined transgenic mice overexpressing angiotensinogen exclusively in the heart. These animals are characterized by high cardiac angiotensin II levels and cardiac hypertrophy accompanied or not by high blood pressure depending on their genetic background, i.e. presence of one or two renin genes. Alpha-skeletal actin levels were highly increased in transgenic compared to wild-type myocardium independently of the number of renin genes, indicating that angiotensin II can stimulate alpha-skeletal actin expression in normotensive animals. Additional in vitro experiments using cultured mouse and rat cardiomyocytes showed that angiotension II not only increases alpha-skeletal actin expression but also induces an increase of its incorporation within II-bands compared to control cardiomyocytes. Angiotensin II increases also the expression of alpha-smooth muscle actin in sarcomeres of cardiomyocytes as well as in fibroblastic cells present within the culture.
Keywords Actins/ metabolism — Angiotensin II/ physiology — Animals — Base Sequence — Cell Differentiation/ physiology — DNA Primers — Hypertension/ metabolism — Mice — Mice, Transgenic — Myocardium/cytology/ metabolism — Rats — Rats, Wistar — Renin/genetics
Identifiers
PMID: 11776396
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Article - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Citation
(ISO format) 		CLEMENT, Sophie et al. Angiotensin II stimulates alpha-skeletal actin expression in cadiomyocytes in vitro and in vivo in the absence of hypertension. In: Differentiation, 2001, vol. 69, n° 1, p. 66-74. https://archive-ouverte.unige.ch/unige:11200

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Deposited on : 2010-08-27

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