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Identification and characterization of a new source of adult human neural progenitors

Behnan, Jinan
Stangeland, Biljana
Langella, Tiziana
Finocchiaro, Gaetano
Tringali, Giovanni
Murrell, Wayne
Published in Cell Death & Disease. 2017, vol. 8, no. 8, p. e2991
Abstract Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. These cells have been traditionally isolated from hippocampus, subventricular zone and white matter. However, there is still a need for an easily accessible source with better yield to counter the limitations of small surgical samples of previously characterized aNPCs. Here we show that ultrasonic aspirate (UA) samples currently considered as 'biological waste after surgery,' offer a good source for aNPCs. Furthermore, we show that culture conditions dictated the phenotype of cells across patients. The neurosphere-enriched cells were more similar to freshly isolated brain cells, while cells expanded adherently in serum conditions were similar to mesenchymal stem cells. However, cells expanded in these adherent conditions expressed some NPC and glial markers in addition to active canonical Wnt signaling. This suggests a mesenchymal-neuroectodermal hybrid nature of these cells. Finally, we show that UA-NPCs are comparable to those from neurogenic regions. Our findings suggest that UA samples can be used as a source for fresh and in vitro propagated aNPCs that could have various clinical applications.
Keywords Adult Stem Cells/cytology/metabolismCell Differentiation/physiologyCells, CulturedFlow CytometryHippocampus/cytologyHumansLateral Ventricles/cytologyNeural Stem Cells/cytology/metabolismNeurogenesis/physiologyWhite Matter/cytologyWnt Signaling Pathway/physiology
PMID: 28796246
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Research group Groupe Schaller Karl Lothard (neurochirurgie) (851)
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BEHNAN, Jinan et al. Identification and characterization of a new source of adult human neural progenitors. In: Cell Death & Disease, 2017, vol. 8, n° 8, p. e2991. doi: 10.1038/cddis.2017.368 https://archive-ouverte.unige.ch/unige:111994

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Deposited on : 2018-12-05

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