UNIGE document Scientific Article
previous document  unige:11195  next document
add to browser collection

Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation

Neuville, P.
Rensen, Sander
Redard, M.
Van Eys, Guillaume
Published in Circulation research. 1999, vol. 85, no. 1, p. 99-107
Abstract Arterial intimal thickening after endothelial injury induced in rodents has proven to be a relatively unreliable model of restenosis for testing clinically useful compounds. The same has been found for cultured rat or rabbit vascular smooth muscle cells (SMCs). To test alternative possibilities, we have studied several differentiation features of porcine coronary artery SMCs, cultured up to the 5th passage after enzymatic digestion of the media. The effects of heparin, transforming growth factor (TGF)-beta1 or TGF-beta2, and all-trans-retinoic acid (tRA) on proliferation, migration, and differentiation of these cells also were examined. Porcine arterial SMCs in culture not only express high levels of alpha-smooth muscle (SM) actin but, contrary to rodent SMCs, also maintain an appreciable expression of SM myosin heavy chain isoforms 1 and 2, desmin, and smoothelin, a recently described late differentiation marker of vascular SMCs. We demonstrate for the first time that smoothelin is colocalized with alpha-SM actin in these cells. Finally, we show that in the porcine model, heparin is more potent than TGF-beta1 or TGF-beta2 and tRA in terms of inhibition of proliferation and migration and of increasing the expression of differentiation markers. This model should be a useful complement to in vivo studies of SMC differentiation and of pathological situations such as restenosis and atheromatosis.
Keywords AnimalsArteries/cytologyBiological MarkersCell Differentiation/physiologyCells, CulturedCoronary Vessels/ cytologyHeparin/pharmacologyMuscle, Smooth, Vascular/ cytologySwineTransforming Growth Factor beta/pharmacologyTretinoin/pharmacology
PMID: 10400915
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://circres.ahajournals.org/cgi/reprint/85/1/99.pdf
(ISO format)
CHRISTEN, Thomas et al. Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation. In: Circulation research, 1999, vol. 85, n° 1, p. 99-107. doi: 10.1161/01.res.85.1.99 https://archive-ouverte.unige.ch/unige:11195

500 hits



Deposited on : 2010-08-27

Export document
Format :
Citation style :