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Bronchoalveolar cytology for diagnosing pulmonary GVHD after bone marrow transplant in children

Kumar, N.
Ozsahin, H.
Published in Pediatric Pulmonology. 2008, vol. 43, no. 7, p. 697-702
Abstract BACKGROUND: Cytological composition of bronchoalveolar lavage (BAL) fluid in pediatric bone marrow transplant (BMT) recipients with pulmonary complications has not been comprehensively described and BAL specific markers of pulmonary GVHD are lacking. The aim of this retrospective study was to assess the role of BAL in the diagnosis of pulmonary GVHD by comparing BAL cytological findings between pediatric allogenic BMT patients with pulmonary complications and oncology children receiving chemotherapy alone. METHODS: Retrospective analysis of BAL specimens for cytology, total and differential cell counts and presence of infections. RESULTS: Seventeen BMT and 13 chemotherapy BAL were analyzed. BAL total cell count was increased but similar between groups (96.9 x 10(4) vs. 98.2 x 10(4), P = NS). BAL cellular composition differed considerably between groups with a significantly higher number of lymphocytes (18% vs. 6.25%, P = 0.03) and a significantly lower number of neutrophils (25.9% vs. 58%, P = 0.02) in BMT BAL specimens. Atypical epithelial cells were significantly more frequent (75% vs. 30.8%, P = 0.027), and significantly more severe (P = 0.01) in BMT patients. The presence and severity of atypia was not associated with infection or pneumotoxic drug exposure (P = NS). CONCLUSION: BAL cytology differs significantly between BMT and chemotherapy patients. The presence BAL lymphocytosis and severe epithelial cell atypia concomitantly to respiratory symptoms and GVHD in other organs may suggest the diagnosis of pulmonary GHVD. Prospective studies assessing the reliability of this finding combined with markers such as epithelial cell apoptosis and increased cytokines are needed.
Keywords AdolescentBiological MarkersBone Marrow Transplantation/adverse effectsBronchoalveolar Lavage Fluid/cytologyChildEpithelial Cells/pathologyFemaleGraft vs Host Disease/pathologyHumansLung/pathologyLymphocytosisMaleRetrospective Studies
PMID: 18500739
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Research groups Facteurs influençants le développement pulmonaire: étude translationnelle chez l'animal et l'homme (182)
Groupe Laurent Kaiser (virologie clinique) (668)
Groupe Pache Jean-Claude (pathologie clinique) (658)
Recherche en infectiologie pédiatrique et en pédiatrie générale (853)
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ROCHAT, Isabelle et al. Bronchoalveolar cytology for diagnosing pulmonary GVHD after bone marrow transplant in children. In: Pediatric Pulmonology, 2008, vol. 43, n° 7, p. 697-702. doi: 10.1002/ppul.20844 https://archive-ouverte.unige.ch/unige:1119

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Deposited on : 2009-03-09

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