The original checkelist of mosses for Paraguay listed 137 species for the country, based on literature reports. An updated checklist has been compiled here, with corrections and additional names added from recent literature records, and in some cases from herbarium records. A total of 240 moss species, including 8 infraspecific taxa, from 94 genera and 38 families are now recorded for Paraguay. This total includes 8 published nomina nuda and 9 taxa that are listed as present in the country but which lack any specific locality reference. Forty-three taxa are apparent endemics to Paraguay and warrant further taxonomic evaluation. The 240 taxa now recorded for Paraguay are listed here along with relevant bibliographical or locality references. A supplementary list of synonyms is also included.