UNIGE document Scientific Article
previous document  unige:11174  next document
add to browser collection

Regulatory volume increase is associated with p38 kinase-dependent actin cytoskeleton remodeling in rat kidney MTAL

Bustamante, Mauro
Bochaton-Piallat, Marie-Luce
Published in American Journal of Physiology - Renal Physiology. 2003, vol. 285, no. 2, p. F336-347
Abstract The kidney medulla is physiologically exposed to variations in extracellular osmolality. In response to hypertonic cell shrinkage, cells of the rat kidney medullary thick ascending limb of Henle's loop undergo p38 kinase-dependent regulatory volume increase (RVI). In the present study, we investigated the role of actin cytoskeleton reorganization in this process. Addition of hyperosmotic NaCl or sucrose, which activates MAP kinases and reduces cellular volume, induced a sustained actin polymerization occurring after 10 min and concurrently with RVI. In contrast, hyperosmotic urea, which does not modify MAP kinase activity and cellular volume, did not induce sustained actin polymerization. Fluorescence microscopy revealed that hyperosmotic NaCl and sucrose, but not urea, induced the redistribution of F-actin from a dense cortical ring to a diffuse network of actin bundles. Stabilization of actin filaments by jasplakinolide and inhibition of the generation of new actin filaments by swinholide A prevented RVI, whereas depolymerization of actin filaments by latrunculin B attenuated cell shrinkage and enhanced RVI. These actin-interfering drugs did not alter extracellular regulated kinase and p38 kinase activation under hypertonic conditions. Similar to swinholide A, inhibiting p38 kinase with SB-203580 abolished sustained actin polymerization, actin redistribution, and decreased RVI efficacy. We therefore propose that in rat kidney the medullary thick ascending limb of Henle's loop exposed to extracellular hypertonicity, p38 kinase activation induces depolymerization of the F-actin cortical ring and polymerization of a dense diffuse F-actin network that both contribute to increase RVI efficacy.
Keywords Actins/metabolismAnimalsEnzyme Inhibitors/pharmacologyImidazoles/pharmacologyLoop of Henle/ enzymologyMaleMarine Toxins/pharmacologyMicrofilaments/ metabolismMitogen-Activated Protein Kinases/antagonists & inhibitors/ metabolismOsmotic PressurePolymersPyridines/pharmacologyRatsRats, WistarSaline Solution, Hypertonic/pharmacologySucrose/pharmacologyUrea/pharmacologyWater-Electrolyte Balance/drug effects/ physiologyP38 Mitogen-Activated Protein Kinases
PMID: 12724128
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://ajprenal.physiology.org/cgi/reprint/285/2/F336.pdf
(ISO format)
BUSTAMANTE, Mauro et al. Regulatory volume increase is associated with p38 kinase-dependent actin cytoskeleton remodeling in rat kidney MTAL. In: American Journal of Physiology - Renal Physiology, 2003, vol. 285, n° 2, p. F336-347. doi: 10.1152/ajprenal.00003.2003 https://archive-ouverte.unige.ch/unige:11174

462 hits

0 download


Deposited on : 2010-08-27

Export document
Format :
Citation style :