en
Scientific article
English

Cultured arterial smooth muscle cells maintain distinct phenotypes when implanted into carotid artery

Published inArteriosclerosis, thrombosis, and vascular biology, vol. 21, no. 6, p. 949-954
Publication date2001
Abstract

Cultured arterial smooth muscle cells (SMCs) with distinct phenotypic features have been described by several laboratories; however, it is not presently known whether this phenotypic heterogeneity can be maintained within an in vivo environment. To answer this question, we have seeded into the intima of denuded rat carotid artery 2 SMC populations with well-established distinct biological features, ie, spindle-shaped, not growing in the absence of serum, and well differentiated versus epithelioid, growing in the absence of serum, and relatively undifferentiated, derived from the aortic media of newborn rats (aged 4 days) and old rats (aged >18 months), respectively. We show that these 2 populations maintain their distinct biochemical features (ie, expression of alpha-smooth muscle actin, smooth muscle myosin heavy chains, and cellular retinol binding protein-1) in the in vivo environment. The old rat media-derived SMCs continue to produce cellular retinol binding protein-1 but little alpha-smooth muscle actin and smooth muscle myosin heavy chains, whereas the newborn rat media-derived SMCs continue to express alpha-smooth muscle actin and smooth muscle myosin heavy chains but no cellular retinol binding protein-1. Our results reinforce the notion of arterial SMC phenotypic heterogeneity and suggest that in our model, heterogeneity is controlled genetically and not by the local environment.

Keywords
  • Actins/metabolism
  • Animals
  • Animals, Newborn
  • Arteries/ cytology
  • Arteriosclerosis/metabolism/surgery
  • Carotid Artery Injuries/metabolism/ surgery
  • Cells, Cultured
  • Male
  • Muscle, Smooth, Vascular/metabolism/ transplantation
  • Myosin Heavy Chains/metabolism
  • Phenotype
  • Rats
  • Rats, Inbred F344
  • Retinol-Binding Proteins/metabolism
  • Retinol-Binding Proteins, Cellular
Citation (ISO format)
BOCHATON-PIALLAT, Marie-Luce et al. Cultured arterial smooth muscle cells maintain distinct phenotypes when implanted into carotid artery. In: Arteriosclerosis, thrombosis, and vascular biology, 2001, vol. 21, n° 6, p. 949–954. doi: 10.1161/01.atv.21.6.949
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ISSN of the journal1079-5642
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