UNIGE document Preprint
previous document  unige:111536  next document
add to browser collection
Title

Effects of selective serotonin-reuptake inhibitors (SSRIs) in JEG-3 and HIPEC cell models of the extravillous trophoblast

Authors
Clabault, Hélène
Vaillancourt, Cathy
Sanderson, J. Thomas
Submitted to Placenta. 2018
Abstract Introduction Between 2 and 10% of pregnant women are treated with selective serotonin-reuptake inhibitors (SSRIs) for depression. The extravillous trophoblasts (evTBs), which migrate and invade maternal tissues, are crucial for embryo implantation and remodeling of maternal spiral arteries. Poor migration/invasion of evTBs can cause serious pregnancy complications, yet the effects of SSRIs on these processes has never been studied. To determine the effects of five SSRIs (fluoxetine, norfluoxetine, citalopram, sertraline and venlafaxine) on migration/invasion, we used JEG-3 and HIPEC cells as evTB models. Methods Cells were treated with increasing concentrations (0.03–10 μM) of SSRIs. Cell proliferation was monitored using an impedance-based system and cell cycle by flow cytometry. Migration was determined using a scratch test and metalloproteinase (MMP) activities by zymography. Invasion markers were determined by RT-qPCR. Results Fluoxetine and sertraline (10 μM) significantly decreased cell proliferation by 94% and by 100%, respectively, in JEG-3 cells and by 58.6% and 100%, respectively, in HIPEC cells. Norfluoxetine increased MMP-9 activity in JEG-3 cells by 2.0% at 0.03 μM and by 43.9% at 3 μM, but decreased MMP-9 activity in HIPEC cells by 63.7% at 3 μM. Sertraline at 0.03 μM increased mRNA level of TIMP-1 in JEG-3 cells by 36% and that of ADAM-10 by 85% and 115% at 0.3 and 3 μM, respectively. In HIPEC cells, venlafaxine at 0.03 and 0.3 μM, increased ADAM-10 mRNA levels by 156% and 167%, respectively. Discussion This study shows that SSRIs may affect evTBs homeostasis at therapeutic levels and provides guidance for future research.
Keywords HumanPlacentaMigrationZymographyProliferationMatrix metalloproteinasesCell cycleJEG-3HIPEC
Identifiers
Full text
Structures
Research group Laboratoire de biologie des tumeurs gynécologiques et du développement (927)
Citation
(ISO format)
CLABAULT, Hélène et al. Effects of selective serotonin-reuptake inhibitors (SSRIs) in JEG-3 and HIPEC cell models of the extravillous trophoblast. Submitted to: Placenta, 2018. https://archive-ouverte.unige.ch/unige:111536

43 hits

0 download

Update

Deposited on : 2018-11-28

Export document
Format :
Citation style :