Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

Belin, Dominique; Hedgpeth, J.; Selzer, G. B.; Epstein, Richard

doi:10.1073/pnas.76.2.700

Scientific article

English

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

ContributorsBelin, Dominique; Hedgpeth, J.; Selzer, G. B.; Epstein, Richard

Published inProceedings of the National Academy of Sciences of the United States of America, vol. 76, no. 2, p. 700-704

Publication date1979

Abstract

We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.

Keywords

Base Sequence
Binding Sites
Codon
Coliphages/ genetics
Genes, Viral
Mutation
Peptide Chain Initiation, Translational
RNA, Messenger/genetics
Ribosomes/metabolism
Temperature

Affiliation entities

Not a UNIGE publication

Citation (ISO format)

BELIN, Dominique et al. Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4. In: Proceedings of the National Academy of Sciences of the United States of America, 1979, vol. 76, n° 2, p. 700–704. doi: 10.1073/pnas.76.2.700

Article

Identifiers

PID : unige:11142
DOI : 10.1073/pnas.76.2.700
PMID : 284393

Additional URL for this publicationhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC383023/pdf/pnas00002-0158.pdf

Journal ISSN0027-8424

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Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4

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