en
Scientific article
English

JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells

Published inThe Journal of biological chemistry, vol. 276, no. 4, p. 2733-2741
Publication date2001
Abstract

Cell-cell contacts are essential for morphogenesis and tissue function and play a vital role in mediating endothelial cohesion within the vascular system during vessel growth and organization. We identified a novel junctional adhesion molecule, named JAM-2, by a selective RNA display method, which allowed identification of transcripts encoding immunoglobulin superfamily molecules regulated during coculture of endothelial cells with tumor cells. The JAM-2 transcript is highly expressed during embryogenesis and is detected in lymph node and Peyer's patches RNA of adult mice. Accordingly, antibodies specific for JAM-2 stain high endothelial venules and lymphatic vessels in lymphoid organs, and vascular structures in the kidney. Using real time video microscopy, we show that JAM-2 is localized within minutes to the newly formed cell-cell contact. The role of the protein in the sealing of cell-cell contact is further suggested by the reduced paracellular permeability of cell monolayer transfected with JAM-2 cDNA, and by the localization of JAM-2 to tight junctional complexes of polarized cells. Taken together, our results suggest that JAM-2 is a novel vascular molecule, which participates in interendothelial junctional complexes.

Keywords
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules/genetics/ isolation & purification
  • Cell Compartmentation
  • Cell Polarity
  • Endothelium, Lymphatic/ chemistry/ultrastructure
  • Endothelium, Vascular/ chemistry/ultrastructure
  • Immunoglobulins/genetics/ isolation & purification
  • Kidney/blood supply
  • Membrane Proteins/genetics/ isolation & purification
  • Mice
  • Molecular Sequence Data
  • Receptors, Cell Surface
  • Sequence Homology, Amino Acid
  • Tight Junctions/ chemistry/ultrastructure
  • Tissue Distribution
Affiliation Not a UNIGE publication
Citation (ISO format)
AURRAND-LIONS, Michel et al. JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. In: The Journal of biological chemistry, 2001, vol. 276, n° 4, p. 2733–2741. doi: 10.1074/jbc.M005458200
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Identifiers
ISSN of the journal0021-9258
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