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In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system

Petit-Pierre, Guillaume
Colin, Philippe
Bertsch, Arnaud
Schneider, Bernard L
Renaud, Philippe
Published in Nature Communications. 2017, vol. 8, no. 1, p. 1239
Abstract Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
PMID: 29093476
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Article (Published version) (1.4 MB) - public document Free access
Research group Toxicologie analytique et forensique (120)
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PETIT-PIERRE, Guillaume et al. In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system. In: Nature Communications, 2017, vol. 8, n° 1, p. 1239. https://archive-ouverte.unige.ch/unige:111133

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Deposited on : 2018-11-21

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