UNIGE document Scientific Article
previous document  unige:111005  next document
add to browser collection
Title

Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature

Authors
Essaidi-Laziosi, Manel
Pérez-Rodríguez, Francisco J
show hidden authors show all authors [1 - 11]
Published in PLOS pathogens. 2018, vol. 14, no. 4, e1006962
Abstract Despite their genetic similarities, enteric and respiratory enteroviruses (EVs) have highly heterogeneous biophysical properties and cause a vast diversity of human pathologies. In vitro differences include acid sensitivity, optimal growth temperature and tissue tropism, which reflect a preferential in vivo replication in the respiratory or gastrointestinal tract and are thus key determinants of EV virulence. To investigate the underlying cause of these differences, we generated chimeras at the capsid-level between EV-D68 (a respiratory EV) and EV-D94 (an enteric EV). Although some chimeras were nonfunctional, EV-D94 with both the capsid and 2A protease or the capsid only of EV-D68 were both viable. Using this latter construct, we performed several functional assays, which indicated that capsid proteins determine acid sensitivity and tropism in cell lines and in respiratory, intestinal and neural tissues. Additionally, capsid genes were shown to also participate in determining the optimal growth temperature, since EV-D94 temperature adaptation relied on single mutations in VP1, while constructs with EV-D68 capsid could not adapt to higher temperatures. Finally, we demonstrate that EV-D68 maintains residual binding-capacity after acid-treatment despite a loss of infectivity. In contrast, non-structural rather than capsid proteins modulate the innate immune response in tissues. These unique biophysical insights expose another layer in the phenotypic diversity of one of world's most prevalent pathogens and could aid target selection for vaccine or antiviral development.
Keywords Acids/chemistryCapsid Proteins/genetics/metabolismEnterovirus/classification/physiologyEnterovirus Infections/genetics/metabolism/virologyHumansIntestines/virologyNeurons/virologyRespiratory System/virologyTemperatureViral Tropism
Identifiers
PMID: 29630666
Full text
Article (Published version) (11.9 MB) - public document Free access
Structures
Research groups Groupe Laurent Kaiser (virologie clinique) (668)
Identification des facteurs clés impliqués dans la pathogénèse des rhinovirus et entérovirus (953)
Radicaux libres et cellules souches embryonnaires (60)
Virologie moléculaire (275)
Citation
(ISO format)
ROYSTON, Lena et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. In: PLOS Pathogens, 2018, vol. 14, n° 4, p. e1006962. doi: 10.1371/journal.ppat.1006962 https://archive-ouverte.unige.ch/unige:111005

352 hits

187 downloads

Update

Deposited on : 2018-11-20

Export document
Format :
Citation style :