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Scientific article
English

Mitochondrial localization of glucocortocoid receptor in glial (Muller) cells in the salamander retina

Contributors

Psarra, A. M.; Bochaton-Piallat, Marie-Luce; Gabbiani, Giulio; Sekeris, C. E.; Tsacopoulos, Marcos

Published in

GLIA, vol. 41, no. 1, p. 38-49

Publication date

2003

Abstract

Glucocorticoid hormones regulate the transcription of nuclear genes by way of their receptors. In addition, these hormones modulate mitochondrial gene transcription by mechanisms that remain poorly understood. Using immunofluorescence labeling in isolated Muller and photoreceptor cells and in intact salamander retina, we found that the glucocorticoid receptor (GR) is localized in both cell types. Confocal laser scanning microscopy and double staining with cytochrome oxidase (COX) showed that GR is localized in the mitochondria of Muller cells, but not in the mitochondria of photoreceptors. GR also colocalizes with glutamine synthetase (GS) in the cytoplasm of Muller cells. GR is also localized in the microvilli of the distal process of Muller cells and in the synaptic terminal of photoreceptors. Pre-incubation of Muller cells with 1 microM dexamethasone (DEX) for 7 h led to greater than 50% inhibition of the glutamate-induced increase in mitochondrial NADH. This late effect of glucocorticoids on glutamate metabolism could be ascribed, in part, to a direct action of steroid hormones on mitochondrial metabolism.

Keywords

  • Ambystoma
  • Animals
  • Cells, Cultured
  • Dexamethasone/pharmacology
  • Glucocorticoids/pharmacology
  • Glutamic Acid/metabolism/pharmacology
  • Immunohistochemistry
  • Liver/cytology/metabolism
  • Mitochondria/ metabolism
  • Neuroglia/ cytology/drug effects/ metabolism
  • Photoreceptor Cells, Vertebrate/cytology/metabolism
  • Receptors, Glucocorticoid/ biosynthesis
  • Retina/ cytology

UNIGE affiliation entities

Citation (ISO format)

PSARRA, A. M. et al. Mitochondrial localization of glucocortocoid receptor in glial (Muller) cells in the salamander retina. In: GLIA, 2003, vol. 41, n° 1, p. 38–49. doi: 10.1002/glia.10130

Main files (1)

Article

Identifiers

Journal ISSN

0894-1491
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Technical informations

Creation

27/08/2010 13:33:35

First validation

27/08/2010 13:33:35

Update

14/03/2023 16:02:14

Status update

14/03/2023 16:02:14

Last indexation

29/01/2025 17:08:35
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