Scientific article
Open access

Mycobacterial lipoarabinomannans modulate cytokine production in human T helper cells by interfering with raft/microdomain signalling

Published inCellular and molecular life sciences, vol. 62, no. 2, p. 179-187
  • Open Access - Licence nationale Springer
Publication date2005

Lipoarabinomannans (LAMs) are major lipoglycans of the mycobacterial envelope and constitute immunodominant epitopes of mycobacteria. In this paper, we show that mannose-capped (ManLAM) and non-mannose-capped (PILAM) mycobacterial lipoglycans insert into T helper cell rafts without apparent binding to known receptors. T helper cells modified by the insertion of PILAM responded to CD3 cross-linking by decreasing type 1 (IL-2 and IFN-gamma) and increasing type 2 (IL-4 and IL-5) cytokine production. Modification by the mannose-capped ManLAMs had similar, but more limited effects on T helper cell cytokine production. When incorporated into isolated rafts, PILAMs modulated membrane-associated kinases in a dose-dependent manner, inducing increased phosphorylation of Src kinases and Cbp/PAG in Th1 rafts, while decreasing phosphorylation of the same proteins in Th2 rafts. Mycobacterial lipoglycans thus modify the signalling machineries of rafts/microdomains in T helper cells, a modification of the membrane organization that eventually leads to an overall enhancement of type 2 and inhibition of type 1 cytokine production.

  • Base Sequence
  • Cytokines/ biosynthesis
  • Humans
  • Interleukins/metabolism
  • Lipopolysaccharides/ pharmacology
  • Mannose/chemistry/metabolism
  • Membrane Glycoproteins/chemistry/metabolism
  • Membrane Microdomains/chemistry/ metabolism
  • Molecular Sequence Data
  • Mycobacterium/chemistry/ immunology
  • Signal Transduction
  • Th1 Cells/chemistry/ immunology
  • Th2 Cells/chemistry/ immunology
Affiliation Not a UNIGE publication
Citation (ISO format)
SHABAANA, A. K. et al. Mycobacterial lipoarabinomannans modulate cytokine production in human T helper cells by interfering with raft/microdomain signalling. In: Cellular and molecular life sciences, 2005, vol. 62, n° 2, p. 179–187. doi: 10.1007/s00018-004-4404-5
Main files (1)
Article (Published version)
ISSN of the journal1420-682X

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