

Other version: http://www.springerlink.com/content/377lj5c7djnxb1qx/
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Mycobacterial lipoarabinomannans modulate cytokine production in human T helper cells by interfering with raft/microdomain signalling |
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Authors | ||
Published in | Cellular and molecular life sciences. 2005, vol. 62, no. 2, p. 179-187 | |
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Open Access - Licence nationale Springer |
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Abstract | Lipoarabinomannans (LAMs) are major lipoglycans of the mycobacterial envelope and constitute immunodominant epitopes of mycobacteria. In this paper, we show that mannose-capped (ManLAM) and non-mannose-capped (PILAM) mycobacterial lipoglycans insert into T helper cell rafts without apparent binding to known receptors. T helper cells modified by the insertion of PILAM responded to CD3 cross-linking by decreasing type 1 (IL-2 and IFN-gamma) and increasing type 2 (IL-4 and IL-5) cytokine production. Modification by the mannose-capped ManLAMs had similar, but more limited effects on T helper cell cytokine production. When incorporated into isolated rafts, PILAMs modulated membrane-associated kinases in a dose-dependent manner, inducing increased phosphorylation of Src kinases and Cbp/PAG in Th1 rafts, while decreasing phosphorylation of the same proteins in Th2 rafts. Mycobacterial lipoglycans thus modify the signalling machineries of rafts/microdomains in T helper cells, a modification of the membrane organization that eventually leads to an overall enhancement of type 2 and inhibition of type 1 cytokine production. | |
Keywords | Base Sequence — Cytokines/ biosynthesis — Humans — Interleukins/metabolism — Lipopolysaccharides/ pharmacology — Mannose/chemistry/metabolism — Membrane Glycoproteins/chemistry/metabolism — Membrane Microdomains/chemistry/ metabolism — Molecular Sequence Data — Mycobacterium/chemistry/ immunology — Signal Transduction — Th1 Cells/chemistry/ immunology — Th2 Cells/chemistry/ immunology | |
Identifiers | PMID: 15666089 | |
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![]() ![]() Other version: http://www.springerlink.com/content/377lj5c7djnxb1qx/ |
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Citation (ISO format) | SHABAANA, A. K. et al. Mycobacterial lipoarabinomannans modulate cytokine production in human T helper cells by interfering with raft/microdomain signalling. In: Cellular and molecular life sciences, 2005, vol. 62, n° 2, p. 179-187. doi: 10.1007/s00018-004-4404-5 https://archive-ouverte.unige.ch/unige:11069 |