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English

Transfected muscle and non-muscle actins are differentially sorted by cultured smooth muscle and non-muscle cells

Contributors

Mounier, N.; Perriard, J. C.; Gabbiani, Giulio; Chaponnier, Christine

Published in

Journal of cell science, vol. 110, no. 7, p. 839-846

Publication date

1997

Abstract

We have analyzed by immunolabeling the fate of exogenous epitope-tagged actin isoforms introduced into cultured smooth muscle and non-muscle (i.e. endothelial and epithelial) cells by transfecting the corresponding cDNAs in transient expression assays. Exogenous muscle actins did not produce obvious shape changes in transfected cells. In smooth muscle cells, transfected striated and smooth muscle actins were preferentially recruited into stress fibers. In non-muscle cells, exogenous striated muscle actins were rarely incorporated into stress fibers but remained scattered within the cytoplasm and frequently appeared organized in long crystal-like inclusions. Transfected smooth muscle actins were incorporated into stress fibers of epithelial cells but not of endothelial cells. Exogenous non-muscle actins induced alterations of cell architecture and shape. All cell types transfected by non-muscle actin cDNAs showed an irregular shape and a poorly developed network of stress fibers. beta- and gamma-cytoplasmic actins transfected into muscle and non-muscle cells were dispersed throughout the cytoplasm, often accumulated at the cell periphery and rarely incorporated into stress fibers. These results show that isoactins are differently sorted: not only muscle and non-muscle actins are differentially distributed within the cell but also, according to the cell type, striated and smooth muscle actins can be discriminated for. Our observations support the assumption of isoactin functional diversity.

Keywords

  • Actins/ metabolism
  • Animals
  • Cattle
  • Cells, Cultured
  • DNA, Complementary/metabolism
  • Endothelium, Vascular/ metabolism
  • Epithelium/metabolism
  • Fluorescent Antibody Technique, Indirect
  • Immunohistochemistry
  • Muscle, Smooth, Vascular/ metabolism
  • Rats
  • Rats, Wistar
  • Transfection

UNIGE affiliation entities

Citation (ISO format)

MOUNIER, N. et al. Transfected muscle and non-muscle actins are differentially sorted by cultured smooth muscle and non-muscle cells. In: Journal of cell science, 1997, vol. 110, n° 7, p. 839–846.

Main files (1)

Article

Identifiers

Additional URL for this publication

http://jcs.biologists.org/cgi/reprint/110/7/839

Journal ISSN

0021-9533
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448downloads

Technical informations

Creation

27/08/2010 13:33:25

First validation

27/08/2010 13:33:25

Update

14/03/2023 16:02:05

Status update

14/03/2023 16:02:05

Last indexation

29/10/2024 16:48:49
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