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Scientific article
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The determination of huperzine A in european Lycopodiaceae species by HPLC-UV-MS

Published inPhytochemical analysis, vol. 17, no. 5, p. 332-336
Publication date2006
Abstract

A rapid qualitative HPLC-UV-MS method was developed for the detection of huperzine A in Lycopodiaceac species. HPLC coupled with mass spectrometry experiments allowed the identification of the alkaloid and enabled targeted analysis of complex matrices such as herbal extracts. Huperzine A was detected by single ion monitoring of the pseudomolecular ion [M + H](+) at m/z 243.2. The alkaloid was also detected on TLC in a bioautographic enzyme assay with acetylcholinesterase to show the activity of the compound. Four Lycopodiaceae species collected in Switzerland were tested by these methods and Huperzia selago (L.) Bernh. ex Schrank et Martius was the only species found to contain huperzine A. Copyright (c) 2006 John Wiley & Sons, Ltd.

Keywords
  • HPLC-UV-MS
  • TLC
  • Huperzine A
  • Lycopodiaceae
  • Huperzia selago
Citation (ISO format)
BORLOZ, A., MARSTON, Andrew, HOSTETTMANN, Kurt. The determination of huperzine A in european Lycopodiaceae species by HPLC-UV-MS. In: Phytochemical analysis, 2006, vol. 17, n° 5, p. 332–336. doi: 10.1002/pca.922
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ISSN of the journal0958-0344
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