Scientific article

Autoantibody repertoire analysis in normal and lupus-prone mice

Published inJournal of autoimmunity, vol. 2, no. 5, p. 657-674
Publication date1989

We have analyzed at the clonal level (limiting dilution assay) the repertoire of lipopolysaccharide (LPS)-responsive murine B cells committed to the production of autoantibodies characteristic of systemic lupus erythematous (SLE), i.e. anti-single-stranded DNA (ssDNA), anti-double-stranded DNA, anti-Sm and rheumatoid factors (RF). Our results demonstrated that: (1) the frequency of precursor B cells producing each lupus autoantibody (approximately 1 in every 100-400 LPS-responding B cell) was similar in two non-autoimmune (C57BL/6 and BALB/c) and four SLE-prone (NZB, (NZB x NZW)F1, MRL/MpJ and BXSB/MpJ) mice despite the marked differences in autoimmune responses in the different SLE-prone mice, and (2) the relative frequency of autoantibody-secreting precursor B cells was constant throughout life, and equally distributed among activated and resting B-cell populations and among B cells from the peritoneal cavity and spleen. The lack of association of anti-ssDNA secretion with anti-Sm or RF secretion in cultures set up with a smaller number of B cells ruled out the possibility that the similar frequency of different autoantibody-secreting cell precursors is due to the poly-specificity of IgM autoantibodies. Notably, the frequencies of autoantibody-secreting precursor cells were significantly lower, approximately 4 and 10 times, than those of anti-tetanus toxoid and anti-dinitrophenyl antibody-producing precursor B cells, respectively. The similar frequency of precursor B cells producing four different lupus autoantibodies on the one hand and the considerable variation in each autoimmune response among SLE-prone mice on the other, support the hypothesis that specific stimulatory mechanisms may govern each autoimmune response in different SLE strains of mice.

  • Animals
  • Animals, Newborn/immunology
  • Autoantibodies/ analysis/immunology
  • B-Lymphocytes/ immunology
  • Cell Differentiation
  • Clone Cells/immunology
  • Disease Models, Animal
  • Female
  • Lipopolysaccharides/pharmacology
  • Lupus Erythematosus, Systemic/genetics/ immunology
  • Lymphocyte Activation/drug effects
  • Mice
  • Mice, Inbred NZB/ immunology
  • Mice, Inbred Strains/ immunology
  • Mice, Mutant Strains/ immunology
  • Models, Biological
  • Peritoneal Cavity/pathology
  • Rats
  • Rats, Inbred Strains/immunology
  • Spleen/pathology
Affiliation Not a UNIGE publication
Citation (ISO format)
STAROBINSKI, M. et al. Autoantibody repertoire analysis in normal and lupus-prone mice. In: Journal of autoimmunity, 1989, vol. 2, n° 5, p. 657–674.
ISSN of the journal0896-8411

Technical informations

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