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Scientific article
English

Plasmodium falciparum synthesizes O-glycosylated glycoproteins containing O-linked N-acetylglucosamine

Contributors

Nasir ud, Din; Drager-Dayal, R.; Decrind, C.; Hu, B. H.; Del Giudice, Giuseppe; Hoessli, Daniel

Published in

Biochemistry international, vol. 27, no. 1, p. 55-64

Publication date

1992

Abstract

Asexual blood forms of the human malaria parasite, Plasmodium falciparum, synthesize a major glycosylated 195 kDa protein that has been considered for the development of a vaccine. beta-Elimination-borohydride reduction of the 195 kDa glycoprotein and its 16 kDa processed product after metabolic labeling of their carbohydrates, showed the presence of derived, labeled glucosaminitol and alanine. This suggests that the 195 and 16 kDa glycoproteins contain distinct O-glycosyl linkages and that N-acetylglucosamine and serine residues are involved in the attachment of carbohydrate moieties to the protein core. Endo-O-glycanase treatment of total glycoproteins shows that O-glycosidycally-linked sugars represent a major carbohydrate moiety in P. falciparum glycoproteins.

Keywords

  • Acetylglucosamine/chemistry/ metabolism
  • Animals
  • Chromatography, Paper
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins/ biosynthesis/chemistry/metabolism
  • Glycosylation
  • Molecular Structure
  • Plasmodium falciparum/ metabolism

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

NASIR UD, Din et al. Plasmodium falciparum synthesizes O-glycosylated glycoproteins containing O-linked N-acetylglucosamine. In: Biochemistry international, 1992, vol. 27, n° 1, p. 55–64.

Identifiers

Journal ISSN

0158-5231
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Technical informations

Creation

26/08/2010 12:36:38

First validation

26/08/2010 12:36:38

Update

14/03/2023 16:01:39

Status update

14/03/2023 16:01:39

Last indexation

29/10/2024 16:46:10
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