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Title

Lack of anti-DNA precursors in lambda chain-bearing B lymphocytes in (NZB x NZW)F1 mice. Evidence for the contribution of V kappa genes to anti-DNA specificity

Authors
Lacour, M.
Published in European Journal of Immunology. 1991, vol. 21, no. 3, p. 839-842
Abstract We have analyzed by the limiting dilution assay on spleen cells from (NZB x NZW)F1 hybrid mice the repertoire of lipopolysaccharide-responsive murine kappa- and lambda 1-secreting B cells committed to the production of anti-DNA autoantibodies to determine the contribution of the heavy and light chains to anti-DNA specificities. Our results demonstrated that anti-DNA precursors were predominantly found in the kappa-secreting B cell population, but not in lambda 1-secreting B cells, while anti-hapten, dinitrophenyl, and anti-tetanus toxoid activities were distributed fairly well in both populations of B cells. This suggests that the V kappa gene segments are critically involved in the generation of anti-DNA specificities, and that at least at the germ-line gene level, the heavy chain V region genes by themselves are not able to confer the anti-DNA autoreactivity.
Keywords AnimalsAntibodies, Antinuclear/ immunologyAntibody DiversityAntibody SpecificityB-Lymphocytes/ physiologyDinitrobenzenes/immunologyImmunoglobulin Variable Region/ immunologyImmunoglobulin kappa-Chains/ immunologyImmunoglobulin lambda-Chains/ immunologyLipopolysaccharides/pharmacologyMiceMice, Inbred NZB/ immunologyMice, Inbred StrainsSpleen/immunologyTetanus Toxoid/immunology
Identifiers
PMID: 1901268
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LACOUR, M., IZUI, Shozo. Lack of anti-DNA precursors in lambda chain-bearing B lymphocytes in (NZB x NZW)F1 mice. Evidence for the contribution of V kappa genes to anti-DNA specificity. In: European Journal of Immunology, 1991, vol. 21, n° 3, p. 839-842. doi: 10.1002/eji.1830210347 https://archive-ouverte.unige.ch/unige:10936

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Deposited on : 2010-08-26

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