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Scientific article
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Gelsolin-actin complex is target for ADP-ribosylation by Clostridium botulinum C2 toxin in intact human neutrophils

Published inEuropean journal of pharmacology, vol. 246, no. 3, p. 293-297
Publication date1993
Abstract

The gelsolin-actin complex was ADP-ribosylated by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin in lysates of human platelets and human neutrophils. When [32P]orthophosphate-labelled human neutrophils were treated with C. botulinum C2 toxin, [32P]ADP-ribosylated gelsolin-actin was precipitated with anti-gelsolin antibody. Stimulation of neutrophils by formyl-methionine-leucine-phenylalanine decreased the interaction of gelsolin with ADP-ribosylated actin in toxin-treated cells. The data indicate that the gelsolin-actin complex is a pathophysiological substrate for actin-ADP-ribosylating toxins.

Keywords
  • Actins/chemistry/ metabolism
  • Adenosine Diphosphate Ribose/ metabolism
  • Blood Platelets/drug effects/metabolism
  • Botulinum Toxins/ pharmacology
  • Gelsolin/chemistry/ metabolism
  • Humans
  • Neutrophils/ drug effects/metabolism
Affiliation Not a UNIGE publication
Citation (ISO format)
JUST, I. et al. Gelsolin-actin complex is target for ADP-ribosylation by Clostridium botulinum C2 toxin in intact human neutrophils. In: European journal of pharmacology, 1993, vol. 246, n° 3, p. 293–297. doi: 10.1016/0922-4106(93)90045-b
Identifiers
ISSN of the journal0014-2999
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