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Fate of undifferentiated mouse embryonic stem cells within the rat heart: role of myocardial infarction and immune suppression

Publié dansJournal of Cellular and Molecular Medicine, vol. 13, no. 1, p. 188-201
Date de publication2009
Résumé

It has recently been suggested that the infarcted rat heart microenvironment could direct pluripotent mouse embryonic stem cells to differentiate into cardiomyocytes through an in situ paracrine action. To investigate whether the heart can function as a cardiogenic niche and confer an immune privilege to embryonic stem cells, we assessed the cardiac differentiation potential of undifferentiated mouse embryonic stem cells (mESC) injected into normal, acutely or chronically infarcted rat hearts. We found that mESC survival depended on immunosuppression both in normal and infarcted hearts. However, upon Cyclosporin A treatment, both normal and infarcted rat hearts failed to induce selective cardiac differentiation of implanted mESC. Instead, teratomas developed in normal and infarcted rat hearts 1 week and 4 weeks (50% and 100%, respectively) after cell injection. Tight control of ESC commitment into a specific cardiac lineage is mandatory to avoid the risk of uncontrolled growth and tumourigenesis following transplantation of highly plastic cells into a diseased myocardium.

Mots-clés
  • Animals
  • Cell Differentiation/ physiology
  • Cell Lineage
  • Cyclosporine/metabolism
  • Embryonic Stem Cells/cytology/ physiology/ transplantation
  • Humans
  • Immunosuppression
  • Immunosuppressive Agents/metabolism
  • Male
  • Mice
  • Myocardial Infarction/ pathology
  • Myocardium/cytology/pathology
  • Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Teratoma/metabolism/pathology
  • Transplantation, Heterologous
  • Ventricular Function, Left
Citation (format ISO)
HE, Qing et al. Fate of undifferentiated mouse embryonic stem cells within the rat heart: role of myocardial infarction and immune suppression. In: Journal of Cellular and Molecular Medicine, 2009, vol. 13, n° 1, p. 188–201. doi: 10.1111/j.1582-4934.2008.00323.x
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Article (Published version)
Identifiants
ISSN du journal1582-1838
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Informations techniques

Création26.08.2010 12:36:31
Première validation26.08.2010 12:36:31
Heure de mise à jour14.03.2023 16:01:31
Changement de statut14.03.2023 16:01:31
Dernière indexation12.02.2024 11:15:45
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