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Thèse
Accès libre
Anglais

Functional genomics of purple sulfur bacteria from Lake Cadagno

Contributeurs/tricesLudin, Samuel
Directeurs/tricesHothorn, Michael
Collaborateurs/tricesTonolla, Mauro Amedeo
Date de soutenance2018-09-03
Résumé

The meromictic Lake Cadagno (Swiss Alps) is an ideal study site for euxinic microbial communities due to the accessibility and the detailed scientific record spanning from biogeochemistry to biophysics and microbial ecology. The phototrophic sulfur oxidizing bacteria (PSOB) community of Lake Cadgno has been studied for years in situ and strains have been isolated and characterized, however information on genomic level was missing. For the first time, with this study we provide novel ecological insights on the biological functions encoded in two PSOB species preforming whole genome sequencing. We then elucidated in-depth the day/night differences in carbon and energy metabolic pathways of PSB ‘Thiodictyon syntrophicum' nov. sp. str. Cad16T in situ with a combination of quantitative proteomics and radiotracer uptake experiments. Finally we additionally demonstrated the use of genomic data as predictive biomarkers for rapid mass-spectrometry based typing of environmental isolates.

eng
Mots-clés
  • Microbial Sulfur cycling
  • Environmental microbiology
  • Meromixis
  • Proteogenomics
  • Dark carbon fixation
  • Whole genome comparison
Groupe de recherche
Citation (format ISO)
LUDIN, Samuel. Functional genomics of purple sulfur bacteria from Lake Cadagno. 2018. doi: 10.13097/archive-ouverte/unige:108895
Fichiers principaux (1)
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Identifiants
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Informations techniques

Création17/09/2018 16:47:00
Première validation17/09/2018 16:47:00
Heure de mise à jour15/03/2023 08:44:32
Changement de statut15/03/2023 08:44:32
Dernière indexation29/01/2024 21:36:37
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