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Scientific article
English

Real-time quantitative polymerase chain reaction analysis of patients with refractory chronic periodontitis

Published inJournal of Periodontology, vol. 82, no. 7, p. 1018-1024
Publication date2011
Abstract

Periodontitis is a complex multifactorial disease and is typically polygenic in origin. Genes play a fundamental part in each biologic process forming complex networks of interactions. However, only some genes have a high number of interactions with other genes in the network and may, therefore, be considered to play an important role. In a preliminary bioinformatic analysis, five genes that showed a higher number of interactions were identified and termed leader genes. In the present study, we use real-time quantitative polymerase chain reaction (PCR) technology to evaluate the expression levels of leader genes in the leukocytes of 10 patients with refractory chronic periodontitis and compare the expression levels with those of the same genes in 24 healthy patients.

Keywords
  • Adult
  • Aggressive Periodontitis/genetics
  • Chronic Periodontitis/genetics
  • Computational Biology
  • Disease Progression
  • Female
  • GRB2 Adaptor Protein/genetics
  • Gene Expression Regulation/genetics
  • Genetic Predisposition to Disease/genetics
  • Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
  • Humans
  • Male
  • Middle Aged
  • NF-kappa B p50 Subunit/genetics
  • Oncogene Protein v-cbl/genetics
  • Polymerase Chain Reaction/methods
  • Transcription Factor RelA/genetics
Affiliation Not a UNIGE publication
Citation (ISO format)
MARCONCINI, Simone et al. Real-time quantitative polymerase chain reaction analysis of patients with refractory chronic periodontitis. In: Journal of Periodontology, 2011, vol. 82, n° 7, p. 1018–1024. doi: 10.1902/jop.2010.100312
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Article (Published version)
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Identifiers
ISSN of the journal0022-3492
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