UNIGE document Scientific Article
previous document  unige:108348  next document
add to browser collection
Title

Luciferase-induced photoreductive uncaging of small-molecule effectors

Authors
Published in Nature Communications. 2018, vol. 9, no. 3539
Abstract Bioluminescence resonance energy transfer (BRET) is extensively used to study dynamic systems and has been utilized in sensors for studying protein proximity, metabolites, and drug concentrations. Herein, we demonstrate that BRET can activate a ruthenium-based photocatalyst which performs bioorthogonal reactions. BRET from luciferase to the ruthenium photocatalyst is used to uncage effector molecules with up to 64 turnovers of the catalyst, achieving concentrations >0.6 μM effector with 10 nM luciferase construct. Using a BRET sensor, we further demonstrate that the catalysis can be modulated in response to an analyte, analogous to allosterically controlled enzymes. The BRET-induced reaction is used to uncage small-molecule drugs (ibrutinib and duocarmycin) at biologically effective concentrations in cellulo.
Identifiers
Full text
Structures
Research group Groupe Winssinger
Citation
(ISO format)
LINDBERG, Eric et al. Luciferase-induced photoreductive uncaging of small-molecule effectors. In: Nature Communications, 2018, vol. 9, n° 3539. https://archive-ouverte.unige.ch/unige:108348

35 hits

9 downloads

Update

Deposited on : 2018-09-25

Export document
Format :
Citation style :