Activity-guided isolation of antifungal, antibacterial, and antioxidant compounds from the dichloromethane extract of the aerial parts of Eriophorum scheuchzeri led to the isolation of eight flavonoids. Three isoflavones and one flavanone are new natural compounds. The isolation of the bioactive compounds was performed by centrifugal partition chromatography. Fractions and pure compounds were monitored by a direct TLC bioautographic assay. Their structures were elucidated by spectroscopic and chemical methods.