A high-ltra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOFMS) method using in-source collision-induced dissociation (CID) was developed for globally profiling oxylipin-containing galactolipids in Arabidopsis wounded leaves. MS and pseudo-MS/MS spectra were obtained during a single analytical run by switching a lens of the TOFMS transfer optics from low to high voltage. Numerous known galactolipids were observed, and four novel mono- or di-galactosyl monoacylglycerides (MGMGs or DGMGs) containing oxophytodienoic acid (OPDA) or dinor-oxophytodienoic acid (dn-OPDA), esterified respectively at the sn1 and the sn2 positions, were identified. Rapid microisolation of the galactolipids followed by alkaline and enzymatic hydrolyses enabled the release of the esterified oxylipins, which allowed for the unambiguous characterization of the oxylipin-containing monoacylglycerides. Their strong induction in response to wounding indicates that these compounds are probably lysogalactolipids formed from galactosyldiglycerides in the injured tissues.resolution u