UNIGE document Scientific Article
previous document  unige:107104  next document
add to browser collection
Title

Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments

Authors
Noatynska, Anna
Smith, Chris A
McAinsh, Andrew D
Published in Nature Communications. 2018, vol. 9, no. 1, p. 2042
Abstract Kinetochores are multi-protein complexes that power chromosome movements by tracking microtubules plus-ends in the mitotic spindle. Human kinetochores bind up to 20 microtubules, even though single microtubules can generate sufficient force to move chromosomes. Here, we show that high microtubule occupancy at kinetochores ensures robust chromosome segregation by providing a strong mechanical force that favours segregation of merotelic attachments during anaphase. Using low doses of the microtubules-targeting agent BAL27862 we reduce microtubule occupancy and observe that spindle morphology is unaffected and bi-oriented kinetochores can still oscillate with normal intra-kinetochore distances. Inter-kinetochore stretching is, however, dramatically reduced. The reduction in microtubule occupancy and inter-kinetochore stretching does not delay satisfaction of the spindle assembly checkpoint or induce microtubule detachment via Aurora-B kinase, which was so far thought to release microtubules from kinetochores under low stretching. Rather, partial microtubule occupancy slows down anaphase A and increases incidences of lagging chromosomes due to merotelically attached kinetochores.
Identifiers
PMID: 29795284
Full text
Article (Published version) (5.9 MB) - public document Free access
Structures
Research group Groupe Meraldi Patrick (physiologie cellulaire et métabolisme) (922)
Project FNS: 31003A 160006
Citation
(ISO format)
DUDKA, Damian et al. Complete microtubule-kinetochore occupancy favours the segregation of merotelic attachments. In: Nature Communications, 2018, vol. 9, n° 1, p. 2042. https://archive-ouverte.unige.ch/unige:107104

20 hits

2 downloads

Update

Deposited on : 2018-08-16

Export document
Format :
Citation style :