Doctoral thesis
Open access

Regulation of ESCRT endosomal recruitment by the lipid-binding protein ALIX

ContributorsLarios, Jorge
Defense date2018-05-25

The endosomal sorting complexes required for transport (ESCRT) is a multiprotein complex that participates in processes which require membrane deformation, such as cytokinetic abscission, and multivesicular body (MVB) biogenesis. MVBs contain intraluminal vesicles (ILV), the formation of which plays a key role in the delivery of ubiquitinated cargo to lysosomes for degradation. ALIX is an ESCRT-associated protein known to interact with TSG101 and CHMP4, two ESCRTs, and a late endosomal lipid, LBPA. ALIX and LBPA participate in ILV formation, yet the mechanism by which they act is unknown. Here, we show that ALIX recruits ESCRT proteins, mainly CHMP4, to late endosomes, in an LBPA-dependent manner. Additionally, ALIX overexpression induces the endosomal accumulation of ubiquitinated proteins, providing evidence that the sorting of several specific transmembrane proteins depends on ALIX. Altogether, we illustrate the importance of ALIX and LBPA in the recruitment of ESCRTs and in the sorting of specific cargo.

  • Endosome
  • MVB
  • ILV
  • ALIX
Research group
Citation (ISO format)
LARIOS, Jorge. Regulation of ESCRT endosomal recruitment by the lipid-binding protein ALIX. 2018. doi: 10.13097/archive-ouverte/unige:106922
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Technical informations

Creation06/26/2018 2:20:00 PM
First validation06/26/2018 2:20:00 PM
Update time03/15/2023 8:29:18 AM
Status update03/15/2023 8:29:17 AM
Last indexation09/18/2023 9:08:16 PM
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