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In vivo quantification of 5-HT1A-[18F]MPPF interactions in rats using the YAP-(S)PET scanner and a beta-microprobe

Moulin, Marcelle
Bartoli, Antonietta
Del Guerra, Alberto
Lemoucheux, Laurent
Buono, Stephano
Fagret, Daniel
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Published in NeuroImage. 2008, vol. 41, no. 3, p. 823-34
Abstract We performed full modeling analysis of 5-HT(1A)-[(18)F]MPPF interactions using the beta-microprobe (beta P) and a YAP-(S)PET scanner. Sixteen Wistar rats were used for beta P (n=5) and YAP-(S)PET (n=5) acquisitions and metabolite studies (n=6). Time-concentration curves were obtained in the hippocampus, raphe dorsalis, frontal cortex and cerebellum, using three injections of [(18)F]MPPF at different specific activities. B'(max) values were estimated from a two (2T-5k)- and three (3T-7k)-tissue-compartment model with beta P and YAP-(S)PET time-concentration curves. The simplified reference tissue model (SRTM) was used to estimate binding potential (BP(SRTM)) values from data obtained with the first injection and the cerebellum as the reference region. Overall, the 3T-7k model provided a better fit than the 2T-5k model, as evaluated from AIC criteria in all experiments. The rank order of receptor density (B'max) values was as follows: hippocampus>raphe approximately frontal cortex>cerebellum. Non-negligible specific binding was observed in the cerebellum (B'max (beta P)=1.5+/-0.9 pmol/ml). Significant correlations (p<0.001) between B'max and BP(SRTM) values were evident with both beta P (r=0.895) and YAP-(S)PET (r=0.695). The YAP-(S)PET system underestimated the [18F]MPPF binding levels in brain due to limited resolution (i.e. partial volume), but led to similar conclusions.
Keywords AnimalsAutoradiographyBrain/metabolismBrain ChemistryFluorine Radioisotopes/pharmacokineticsMalePositron-Emission TomographyRadiopharmaceuticals/pharmacokineticsRatsRats, WistarReceptor, Serotonin, 5-HT1A/metabolismSerotonin/metabolism
PMID: 18436452
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Article (Accepted version) (1.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research groups Etude de l'anatomie fonctionnelle des processus cognitifs supérieurs (3)
Neuroimagerie moléculaire en psychiatrie (983)
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MILLET, Philippe et al. In vivo quantification of 5-HT1A-[18F]MPPF interactions in rats using the YAP-(S)PET scanner and a beta-microprobe. In: NeuroImage, 2008, vol. 41, n° 3, p. 823-34. doi: 10.1016/j.neuroimage.2008.02.062 https://archive-ouverte.unige.ch/unige:1069

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Deposited on : 2009-03-04

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