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Scientific article
English

Remodeling of synaptic membranes after induction of long-term potentiation

Contributors

Toni, Nicolas; Buchs, Pierre-Alain; Nikonenko, Irina; Povilaitite, P.; Parisi, Lorena; Muller, Dominique

Published in

The Journal of neuroscience, vol. 21, no. 16, p. 6245-6251

Publication date

2001

Abstract

Several morphological changes of synapses have been reported to be associated with the induction of long-term potentiation (LTP) in the CA1 hippocampus, including an transient increase in the proportion of synapses with perforated postsynaptic densities (PSDs) and a later occurrence of multiple spine boutons (MSBs) in which the two spines arise from the same dendrite. To investigate the functional significance of these modifications, we analyzed single sections and reconstructed 134 synapses labeled via activity using a calcium precipitation approach. Analyses of labeled spine profiles showed changes of the spine head area, PSD length, and proportion of spine profiles containing a coated vesicle that reflected variations in the relative proportion of different types of synapses. Three-dimensional reconstruction indicated that the increase of perforated spine profiles observed 30 min after LTP induction essentially resulted from synapses exhibiting segmented, completely partitioned PSDs. These synapses had spine head and PSD areas approximately three times larger than those of simple synapses. They contained coated vesicles in a much higher proportion than that of any other type of synapse and exhibited large spinules associated with the PSD. Also the MSBs with two spines arising from the same dendrite that were observed 1-2 hr after LTP induction included a spine that was smaller and a PSD that was smaller than those of simple synapses. These results support the idea that LTP induction is associated with an enhanced recycling of synaptic membrane and that this process could underlie the formation of synapses with segmented PSDs and eventually result in the formation of a new, immature spine.

Keywords

  • Animals
  • Calcium/metabolism
  • Cell Surface Extensions/metabolism/ ultrastructure
  • Coated Vesicles/ultrastructure
  • Dendrites/metabolism/ultrastructure
  • Electric Stimulation
  • Hippocampus/metabolism/ultrastructure
  • Long-Term Potentiation/ physiology
  • Microscopy, Electron
  • Neuronal Plasticity/physiology
  • Rats
  • Synapses/metabolism/ ultrastructure
  • Synaptic Membranes/metabolism/ ultrastructure

UNIGE affiliation entities

Citation (ISO format)

TONI, Nicolas et al. Remodeling of synaptic membranes after induction of long-term potentiation. In: The Journal of neuroscience, 2001, vol. 21, n° 16, p. 6245–6251.

Main files (1)

Article

Identifiers

Additional URL for this publication

http://www.jneurosci.org/cgi/reprint/21/16/6245.pdf

Journal ISSN

0270-6474
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Technical informations

Creation

06/08/2010 13:47:52

First validation

06/08/2010 13:47:52

Update

14/03/2023 15:59:33

Status update

14/03/2023 15:59:33

Last indexation

29/10/2024 16:34:09
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