The aim of this thesis was to investigate how different physical techniques, in particular iontophoresis and fractional laser ablation, could be used to enhance transdermal and cutaneous drug delivery. First part of the thesis describe how iontophoresis can be used to control transdermal delivery kinetics of drugs for neurodegenerative disorders. Second part of the thesis was focused on to investigate the transdermal iontophoretic delivery of degarelix. Overall, this research highlights the complicated interaction between peptide structure, skin membranes and plasma proteins that must be judiciously considered in order to achieve significant delivery. Last part of the thesis presents a novel approach for creating intraepidermal drug reservoirs using fractional laser ablation to deposit microparticles loaded with either a low molecular weight drug (triamcinolone acetonide) or an antibody (cetuximab) so as to provide localized sustained delivery. The results justify that this combination approach is of particular interest for dermatological conditions.