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Title

Expression of c-ets-1, collagenase 1, and urokinase-type plasminogen activator genes in lung carcinomas

Authors
Gouyer, V
Devouassoux, M
Vandenbunder, B
Wernert, N
Moro, D
Brambilla, C
Brambilla, E
Published in The American Journal of Pathology. 1995, vol. 147, no. 5, p. 1298-1310
Abstract The c-ets-1 transcription factor has been involved in the in vitro transactivation of matrix-degrading protease genes that might play an important role in tumor invasion. Using in situ hybridization, we analyzed serial frozen sections for c-ets-1, collagenase 1, and urokinase-type plasminogen activator gene expression in 54 lung carcinomas including 34 non-neuroendocrine carcinomas (18 squamous carcinomas, 10 adenocarcinomas, 3 large cell carcinomas, and 3 basaloids) and 20 neuroendocrine carcinomas (7 small cell lung carcinomas, 4 large cell neuroendocrine carcinomas, 4 well differentiated neuroendocrine carcinomas, and 5 carcinoids). c-ets-1 gene was expressed in stromal cells in 44/54 lung carcinomas including one metastasizing carcinoid. c-ets-1 transcripts were also detected in cancer cells more frequently in neuroendocrine than in non-neuroendocrine carcinomas (P = 0.0059) and in stages III and IV and metastasis more frequently than in stages I and II ( P = 0.0065). Collagenase 1 gene was expressed in 16/34 non-neuroendocrine tumors and in 1/20 neuroendocrine tumors, either in stromal (12/17) or in cancer cells (6/17). Urokinase-type plasminogen activator mRNAs were expressed in 45/54 lung carcinomas in stromal and/or cancer cells. In non-neuroendocrine tumors, c-ets-1 and collagenase 1 gene expressions in stromal cells were correlated. These results demonstrate that the transcription factor c-ets-1, collagenase 1, and urokinase-type plasminogen activator are involved in lung cancer invasion and suggest that c-ets-1 protein might transactivate collagenase 1 gene during tumor invasion.
Keywords Carcinoma/enzymology/genetics/secondaryCollagenases/geneticsGene Expression Regulation, NeoplasticHumansLung Neoplasms/enzymology/genetics/pathologyNeoplasm StagingProtein-Tyrosine Kinases/geneticsProto-Oncogene Proteins/geneticsProto-Oncogene Proteins c-etsRNA, Messenger/analysisStromal Cells/enzymology/pathologyTranscription Factors/geneticsUrokinase-Type Plasminogen Activator/genetics
Identifiers
PMID: 7485393
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BOLON, Isabelle et al. Expression of c-ets-1, collagenase 1, and urokinase-type plasminogen activator genes in lung carcinomas. In: The American Journal of Pathology, 1995, vol. 147, n° 5, p. 1298-1310. https://archive-ouverte.unige.ch/unige:104580

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Deposited on : 2018-05-22

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