Sildenafil, a cyclic GMP phosphodiesterase inhibitor, induces microglial modulation after focal ischemia in the neonatal mouse brain

Moretti, Raffaella; Leger, Pierre-Louis; Besson, Valérie C; Csaba, Zsolt; Pansiot, Julien; Di Criscio, Lorena; Gentili, Andrea; Titomanlio, Luigi; Bonnin, Philippe; Baud, Olivier; Charriaut-Marlangue, Christiane

doi:10.1186/s12974-016-0560-4

Scientific article

English

Sildenafil, a cyclic GMP phosphodiesterase inhibitor, induces microglial modulation after focal ischemia in the neonatal mouse brain

ContributorsMoretti, Raffaella; Leger, Pierre-Louis; Besson, Valérie C; Csaba, Zsolt; Pansiot, Julien; Di Criscio, Lorena; Gentili, Andrea; Titomanlio, Luigi; Bonnin, Philippe; Baud, Olivier; Charriaut-Marlangue, Christiane

Published inJournal of Neuroinflammation, vol. 13, no. 1, 95

Publication date2016

Abstract

Perinatal ischemic stroke is the most frequent form of cerebral infarction in neonates; however, evidence-based treatments are currently lacking. We have previously demonstrated a beneficial effect of sildenafil citrate, a PDE-5 inhibitor, on stroke lesion size in neonatal rat pups. The present study investigated the effects of sildenafil in a neonatal mouse stroke model on (1) hemodynamic changes and (2) regulation of astrocyte/microglia-mediated neuroinflammation.

Keywords

Animals
Animals, Newborn
Brain Ischemia/enzymology/pathology
Disease Models, Animal
Immunohistochemistry
Mice
Mice, Inbred C57BL
Microglia/drug effects/enzymology
Phosphodiesterase Inhibitors/pharmacology
Polymerase Chain Reaction
Sildenafil Citrate/pharmacology

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Research groups

Prévention des complications néonatales d'origine périnatale (990)

Citation (ISO format)

MORETTI, Raffaella et al. Sildenafil, a cyclic GMP phosphodiesterase inhibitor, induces microglial modulation after focal ischemia in the neonatal mouse brain. In: Journal of Neuroinflammation, 2016, vol. 13, n° 1, p. 95. doi: 10.1186/s12974-016-0560-4