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Title

Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells
Authors
Suva, Domizio
Garavaglia, Guido
Menetrey, Jacques
Chapuis, Bernard
Hoffmeyer, Pierre
Bernheim, Laurent
Kindler, Vincent Lucien
Published in Journal of Cellular Physiology. 2004, vol. 198, no. 1, p. 110-118
Abstract Mesenchymal stem cells (MSC) are considered as potential agents for reconstructive and gene-targeting therapies since they differentiate into various cell-lineages, exhibit an extended survival once injected into a host, and can easily be transfected with engineered DNA. MSC are essentially isolated from hematopoietic bone marrow (BM), a process that is rather invasive and may raise ethical concerns. In an attempt to find an alternative source, we evaluated whether non-hematopoietic (nh)BM recovered from femoral heads of patients undergoing hip arthroplasty contained MSC. Ex vivo, 99% of nhBM cells were CD45(+) leukocytes. After culture, leukocytes were replaced by a homogeneous layer of adherent CD45(-) CD14(-) CD34(-) CD11b(-) CD90(+) HLA-ABC(+) cells. Culture doubling time (mean = 4 days, range 1.6-6.7 days) was not correlated with patient age (27-81 years, n = 16). Amplified cultures supported long-term hematopoiesis, and could be differentiated in vitro into adipocytes and chondrocytes. Moreover, a small fraction of nhBM cells spontaneously expressed MyoD1 and formed myotubes, suggesting that myogenic differentiation also occurred. nhBM contained clonogenic cells whose frequency (1/13,000), doubling time (2.1 days), and maximal amplification (up to 10(6)-fold) were not age-related. All 14 clones analyzed (from five patients, ages 27-78 years) differentiated into at least one mesenchymal lineage, and 66% were bipotential (n = 8/12), or tripotential (n = 2/3). In conclusion, nhBM contains pluripotential mesenchymal progenitors which are similar to hematopoietic BM-derived MSC, and whose biological functions are not altered by aging. Furthermore, if MSC-based therapies hold their promises, nhBM may become the source of choice for responding to the increasing demand for MSC.
Keywords Adipocytes/cytology/metabolism — Adult — Aged — Aged, 80 and over — Antigens, CD45/metabolism — Bone Marrow Cells/cytology/ metabolism — Cell Differentiation/physiology — Cell Division — Cells, Cultured — Chondrocytes/cytology/metabolism — Femur/cytology/metabolism — Hematopoiesis/ physiology — Humans — Mesoderm/ cytology/metabolism — Middle Aged — Myoblasts/metabolism — Phenotype — Pluripotent Stem Cells/cytology/ physiology
Identifiers
PMID: 14584050
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Other version: http://www3.interscience.wiley.com/journal/104557988/abstract
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Faculté de médecine / Section de médecine clinique / Département de chirurgie
Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales
Research groups Thérapie cellulaire et troubles musculo-squelettiques (960)
Culture Primaire de myoblastes humains (208)
Immunité innée non leucocytaire (928)
Citation
(ISO format) 		SUVA, Domizio et al. Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells. In: Journal of Cellular Physiology, 2004, vol. 198, n° 1, p. 110-118. https://archive-ouverte.unige.ch/unige:10454

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Deposited on : 2010-08-06

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