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unige:10423 
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A new interface chamber for the study of mammalian nervous tissue slices |
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| Authors | ||
| Published in | Journal of Neuroscience Methods. 1995, vol. 58, no. 1-2, p. 203-208 | |
| Abstract | We describe a new interface-type chamber for electrophysiological studies in mammalian brain slices. Thermoregulation of the inner recording chamber is achieved using the Peltier effect and a feedback control unit. Between 15 and 40 degrees C, and for perfusion rates from 1 to 5 ml/min, the temperature can be maintained within +/- 0.1 degrees C of the command value; it can also be rapidly and reliably changed. An external bath, heated by a coiled resistor, generates a humidified, oxygenated atmosphere diffusing above the slices. Survival of neuronal tissue is excellent and stable intracellular recordings can be obtained using either sharp or patch-clamp micropipettes. Perfusion solutions can be readily exchanged, rendering this chamber suitable for the study of bath-applied neuroactive compounds. | |
| Keywords | Animals — Brain/physiology — Diffusion Chambers, Culture — Electrophysiology/ instrumentation — Nerve Tissue/ physiology — Neurons/physiology — Patch-Clamp Techniques — Perfusion/instrumentation — Rats — Temperature — Vagus Nerve/physiology | |
| Identifiers | PMID: 7475228 | |
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Article - Limited access to UNIGE Other version: http://www.sciencedirect.com/science/article/pii/016502709400177I |
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| Structures | ||
| Citation (ISO format) | REYNAUD, J. C. et al. A new interface chamber for the study of mammalian nervous tissue slices. In: Journal of Neuroscience Methods, 1995, vol. 58, n° 1-2, p. 203-208. https://archive-ouverte.unige.ch/unige:10423 |
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