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Title

Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS

Authors
Vaezzadeh, Ali R.
Deshusses, Jacques M.P.
Published in Methods in Molecular Biology. 2011, vol. 681, p. 449-458
Abstract Shotgun proteomics is a rapid and near universal strategy to identify proteins in complex protein mixtures. After protein digestion, the resulting peptide mixture is submitted to two orthogonal techniques: peptides are first separated according to their isoelectric point (pI) by isoelectric focusing (IEF) on immobilized pH gradient (IPG); after peptide extraction, they are then separated in the second dimension according to their hydrophobic properties by reverse phase liquid chromatography (RPLC). Finally, they are detected by tandem mass spectrometry (MS/MS) and proteins are matched by means of bioinformatics software.
Keywords Protein identificationPeptidesIsoelectric focusingLiquid chromatographyTandem mass spectrometry
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GEISER, Laurent et al. Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS. In: Protein Chromatography, 2011, vol. 681, p. 449-458. https://archive-ouverte.unige.ch/unige:103673

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Deposited on : 2018-04-19

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