UNIGE document Doctoral Thesis
previous document  unige:103201  next document
add to browser collection
Title

Flow cytometry as a tool to investigate the anoxygenic phototrophic sulfur bacteria coexistence in the chemocline of meromictic Lake Cadagno

Author
Director
Defense Thèse de doctorat : Univ. Genève, 2018 - Sc. 5184 - 2018/02/12
Abstract The meromictic Lake Cadagno is characterized by a compact chemocline with high concentration of anoxygenic phototrophic purple sulfur bacteria (PSB) and green sulfur bacteria (GSB). The co-occurrence of PSB and GSB in the same ecological niche makes the chemocline of Lake Cadagno an ideal system for studying the conditions and consequences of coexistence of photosynthetic bacteria population. In this study, flow cytometry (FCM) was applied as a fast tool to identify metabolic changes due to the production and consumption of inclusion bodies and to follow population dynamics of closely related anoxygenic photosynthetic sulfur bacteria in their natural environment. Through this technique the large-celled PSB Chromatium okenii and GSB Chlorobium populations were reliably separated and identified due to difference in auto-fluorescence and cell size. Moreover, we showed that these dominant taxa share the same ecological niche. Two mechanisms for this coexistence were proposed: population activity alternation and bioconvection.
Identifiers
URN: urn:nbn:ch:unige-1032010
Full text
Thesis (15.1 MB) - public document Free access
Structures
Citation
(ISO format)
DANZA, Francesco. Flow cytometry as a tool to investigate the anoxygenic phototrophic sulfur bacteria coexistence in the chemocline of meromictic Lake Cadagno. Université de Genève. Thèse, 2018. https://archive-ouverte.unige.ch/unige:103201

94 hits

14 downloads

Update

Deposited on : 2018-03-26

Export document
Format :
Citation style :