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Titles listSynthesis and release of an acetylcholine-like compound by human myoblasts and myotubes
Titles listSynthesis and release of an acetylcholine-like compound by human myoblasts and myotubes
Scientific Article
unige:10295 
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Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes |
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| Authors | ||
| Published in | The Journal of Physiology. 1995, vol. 489, no. 3, p. 791-803 | |
| Abstract | 1. Exogenously applied acetylcholine (ACh) is a modulator of human myoblast fusion. Using a chemiluminescent method, we examined whether an endogenous ACh-like compound (ACh-lc) was present in, and released by, pure human myogenic cells. 2. Single, freshly isolated satellite cells and proliferating myoblasts contained 15 and 0.5 fmol ACh-lc, respectively. Cultured myotubes contained ACh-lc as well. Also, ACh-like immunoreactivity was detected in all myogenic cells. 3. Part of the ACh-lc was synthesized by choline acetyltransferase (ChAT), as indicated by the reduction of ACh-lc content when bromoACh was present in the culture medium, and by direct measurements of ChAT activity. Also, ChAT-like immunoreactivity was observed in all myogenic cells. 4. Myoblasts and myotubes released ACh-lc spontaneously by a partially Ca(2+)-dependent mechanism. 5. The application by microperfusion of medium conditioned beforehand by myoblasts (thus presumably containing ACh-lc) onto a voltage-clamped myotube induced inward currents resembling ACh-induced currents in their kinetics, reversal potential, and sensitivity to nicotinic antagonists. 6. In vitro, the spontaneously released ACh-lc promoted myoblast fusion but only in the presence of an anticholinesterase. 7. Our observations indicate that human myogenic cells synthesize and release an ACh-lc and thereby promote the fusion process that occurs in muscle during growth or regeneration. | |
| Keywords | Acetylcholine/ biosynthesis/metabolism — Adolescent — Adult — Cell Fusion/physiology — Cells, Cultured — Chemiluminescent Measurements — Child — Choline O-Acetyltransferase/metabolism — Cholinesterase Inhibitors/pharmacology — Clone Cells — Flow Cytometry — Humans — Immunohistochemistry — Microtubules/ metabolism — Middle Aged — Muscle Proteins/metabolism — Muscle, Skeletal/cytology/ metabolism — Patch-Clamp Techniques — Physostigmine/pharmacology | |
| Identifiers | PMID: 8788943 | |
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| Citation (ISO format) | HAMANN, M. et al. Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes. In: The Journal of Physiology, 1995, vol. 489, n° 3, p. 791-803. https://archive-ouverte.unige.ch/unige:10295 |
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