Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes

Hamann, M.; Chamoin, M. C.; Portalier, P.; Bernheim, Laurent; Baroffio Barbier, Anne; Widmer, H.; Bader, Charles; Ternaux, J. P.

doi:10.1113/jphysiol.1995.sp021092

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Title		Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes
Authors		Hamann, M. Chamoin, M. C. Portalier, P. Bernheim, Laurent Baroffio Barbier, Anne Widmer, H. Bader, Charles Ternaux, J. P.
Published in		The Journal of Physiology. 1995, vol. 489, no. 3, p. 791-803
Abstract		1. Exogenously applied acetylcholine (ACh) is a modulator of human myoblast fusion. Using a chemiluminescent method, we examined whether an endogenous ACh-like compound (ACh-lc) was present in, and released by, pure human myogenic cells. 2. Single, freshly isolated satellite cells and proliferating myoblasts contained 15 and 0.5 fmol ACh-lc, respectively. Cultured myotubes contained ACh-lc as well. Also, ACh-like immunoreactivity was detected in all myogenic cells. 3. Part of the ACh-lc was synthesized by choline acetyltransferase (ChAT), as indicated by the reduction of ACh-lc content when bromoACh was present in the culture medium, and by direct measurements of ChAT activity. Also, ChAT-like immunoreactivity was observed in all myogenic cells. 4. Myoblasts and myotubes released ACh-lc spontaneously by a partially Ca(2+)-dependent mechanism. 5. The application by microperfusion of medium conditioned beforehand by myoblasts (thus presumably containing ACh-lc) onto a voltage-clamped myotube induced inward currents resembling ACh-induced currents in their kinetics, reversal potential, and sensitivity to nicotinic antagonists. 6. In vitro, the spontaneously released ACh-lc promoted myoblast fusion but only in the presence of an anticholinesterase. 7. Our observations indicate that human myogenic cells synthesize and release an ACh-lc and thereby promote the fusion process that occurs in muscle during growth or regeneration.
Keywords		Acetylcholine/ biosynthesis/metabolism — Adolescent — Adult — Cell Fusion/physiology — Cells, Cultured — Chemiluminescent Measurements — Child — Choline O-Acetyltransferase/metabolism — Cholinesterase Inhibitors/pharmacology — Clone Cells — Flow Cytometry — Humans — Immunohistochemistry — Microtubules/ metabolism — Middle Aged — Muscle Proteins/metabolism — Muscle, Skeletal/cytology/ metabolism — Patch-Clamp Techniques — Physostigmine/pharmacology
Identifiers		DOI: 10.1113/jphysiol.1995.sp021092 PMID: 8788943
Full text		Article (Published version) - Free access Other version: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1156848/pdf/jphysiol00306-0172.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales
Citation (ISO format)		HAMANN, M. et al. Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes. In: The Journal of Physiology, 1995, vol. 489, n° 3, p. 791-803. doi: 10.1113/jphysiol.1995.sp021092 https://archive-ouverte.unige.ch/unige:10295