Scientific article
Open access

Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes

Published inJournal of physiology, vol. 489, no. 3, p. 791-803
Publication date1995

1. Exogenously applied acetylcholine (ACh) is a modulator of human myoblast fusion. Using a chemiluminescent method, we examined whether an endogenous ACh-like compound (ACh-lc) was present in, and released by, pure human myogenic cells. 2. Single, freshly isolated satellite cells and proliferating myoblasts contained 15 and 0.5 fmol ACh-lc, respectively. Cultured myotubes contained ACh-lc as well. Also, ACh-like immunoreactivity was detected in all myogenic cells. 3. Part of the ACh-lc was synthesized by choline acetyltransferase (ChAT), as indicated by the reduction of ACh-lc content when bromoACh was present in the culture medium, and by direct measurements of ChAT activity. Also, ChAT-like immunoreactivity was observed in all myogenic cells. 4. Myoblasts and myotubes released ACh-lc spontaneously by a partially Ca(2+)-dependent mechanism. 5. The application by microperfusion of medium conditioned beforehand by myoblasts (thus presumably containing ACh-lc) onto a voltage-clamped myotube induced inward currents resembling ACh-induced currents in their kinetics, reversal potential, and sensitivity to nicotinic antagonists. 6. In vitro, the spontaneously released ACh-lc promoted myoblast fusion but only in the presence of an anticholinesterase. 7. Our observations indicate that human myogenic cells synthesize and release an ACh-lc and thereby promote the fusion process that occurs in muscle during growth or regeneration.

  • Acetylcholine/ biosynthesis/metabolism
  • Adolescent
  • Adult
  • Cell Fusion/physiology
  • Cells, Cultured
  • Chemiluminescent Measurements
  • Child
  • Choline O-Acetyltransferase/metabolism
  • Cholinesterase Inhibitors/pharmacology
  • Clone Cells
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Microtubules/ metabolism
  • Middle Aged
  • Muscle Proteins/metabolism
  • Muscle, Skeletal/cytology/ metabolism
  • Patch-Clamp Techniques
  • Physostigmine/pharmacology
Citation (ISO format)
HAMANN, M. et al. Synthesis and release of an acetylcholine-like compound by human myoblasts and myotubes. In: Journal of physiology, 1995, vol. 489, n° 3, p. 791–803. doi: 10.1113/jphysiol.1995.sp021092
Main files (1)
Article (Published version)
ISSN of the journal0022-3751

Technical informations

Creation08/06/2010 1:46:42 PM
First validation08/06/2010 1:46:42 PM
Update time03/14/2023 3:58:36 PM
Status update03/14/2023 3:58:35 PM
Last indexation10/18/2023 11:01:58 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack