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Scientific article
English

Design of nucleic acid-layered double hydroxide nanohybrids

Published inColloid and Polymer Science, vol. 295, no. 9, p. 1463-1473
Publication date2017
Abstract

We report here on the design of inorganic–organic hybrid materials, which consist of layered double hydroxides (LDHs) as inorganic carrier and short single-stranded nucleic acids (ssDNA) as organic molecules. LDHs were prepared by the co-precipitation method followed by hydrothermal treatment. A model 12-nucleotide-long sequence was immobilized either by ion exchange or covalent grafting. Both the LDH composition and the nucleic acid-to-particle ratio were opti- mized throughout the synthesis to develop highly stable sus- pensions of the hybrid materials. Structural characterization revealed that the covalent attachment of the ssDNA was suc- cessfully achieved via silanization of the LDHs in aqueous suspension. Covalent linkage of the nucleic acids confers to this model nanoparticulate system promising properties and potential for applications as therapeutic agents. Fragments of nucleic acids could be introduced into living cells without release during the delivery process since LDHs slowly dis- solve in the slightly acidic intracellular space.

Keywords
  • Layered double hydroxide
  • Nucleic acid
  • Biohybrid
  • Colloids
  • Nanoparticles
  • Inorganic oxides
  • Colloidal stability
Citation (ISO format)
ÁDOK-SIPICZKI, Mónika et al. Design of nucleic acid-layered double hydroxide nanohybrids. In: Colloid and Polymer Science, 2017, vol. 295, n° 9, p. 1463–1473. doi: 10.1007/s00396-017-4122-9
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ISSN of the journal0303-402X
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