Scientific article

Decreased protein phosphatase 2A activity in hippocampal long-term potentiation

Published inJournal of neurochemistry, vol. 74, no. 2, p. 807-817
Publication date2000

Using autophosphorylated Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) as substrate, we now find that long-term potentian (LTP) induction and maintenance are also associated with a significant decrease in calyculin A-sensitive protein phosphatase (protein phosphatase 2A) activity, without changes in Mg2+-dependent protein phosphatase (protein phosphatase 2C) activity. This decrease in protein phosphatase 2A activity was prevented when LTP induction was inhibited by treatment with calmidazolium or D-2-amino-5-phosphonopentanoic acid. In addition, the application of high-frequency stimulation to 32P-labeled hippocampal slices resulted in increases in the phosphorylation of a 55-kDa protein immunoprecipitated with anti-phosphatase 2A antibodies. Use of a specific antibody revealed that the 55-kDa protein is the B'alpha subunit of protein phosphatase 2A. Following purification of brain protein phosphatase 2A, the B'alpha subunit was phosphorylated by CaM kinase II, an event that led to the reduction of protein phosphatase 2A activity. These results suggest that the decreased activity in protein phosphatase 2A following LTP induction contributes to the maintenance of constitutively active CaM kinase II and to the long-lasting increase in phosphorylation of synaptic components implicated in LTP.

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases/metabolism
  • Enzyme Activation/physiology
  • Hippocampus/chemistry/cytology/enzymology/ physiology
  • Isoenzymes/metabolism
  • Long-Term Potentiation/ physiology
  • Male
  • Neurons/chemistry/metabolism
  • Phosphoprotein Phosphatases/ metabolism
  • Phosphorylation
  • Protein Phosphatase 2
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Extracts/metabolism
Citation (ISO format)
FUKUNAGA, K. et al. Decreased protein phosphatase 2A activity in hippocampal long-term potentiation. In: Journal of neurochemistry, 2000, vol. 74, n° 2, p. 807–817.
Main files (1)
ISSN of the journal0022-3042

Technical informations

Creation08/06/2010 1:46:36 PM
First validation08/06/2010 1:46:36 PM
Update time03/14/2023 3:58:29 PM
Status update03/14/2023 3:58:29 PM
Last indexation02/12/2024 7:03:19 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack